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PCR Detection of Microbial Pathogens pp 185–192Cite as

Detection of Listeria monocytogenes Using a PCR/DNA Probe Assay

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 216))

Abstract

It is only in recent years that Listeria monocytogenes has become regarded as a significant food-borne pathogen. Interest in the organism arose due to several food-borne outbreaks in the early 1980s of listeriosis. The high mortality rate associated with the illness prompted widespread public concern about the pathogen and resulted in health authorities and the food industry initiating programs to control the organism and the disease. Listeriosis is not characterized by a unique set of symptoms, since its course depends on the state of the host. Approximately one-third of human L. monocytogenes infections are associated with pregnant women and their unborn infants, and the other two thirds occur in non-pregnant immunocompromised individuals of all ages. Symptoms caused by the infection during pregnancy are generally only a mild fever in the mother, with or without gastroenteritis or flu-like symptoms, but the consequences for the fetus or newborn are often major or fatal.

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Author information

Authors and Affiliations

  1. The National Diagnostics Centre, BioResearch Ireland, The National University of Ireland, Galway, Ireland

    Louise O’Connor

Authors
  1. Louise O’Connor
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Editor information

Editors and Affiliations

  1. Federal Institute for Health Protection of Consumers and Veterinary Medicine (BgVV), Jena, Germany

    Konrad Sachse (Dr. Rer. Nat.) (Dr. Rer. Nat.)

  2. University of Bern, Bern, Switzerland

    Joachim Frey PhD

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© 2003 Humana Press Inc., Totowa, NJ

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O’Connor, L. (2003). Detection of Listeria monocytogenes Using a PCR/DNA Probe Assay. In: Sachse, K., Frey, J. (eds) PCR Detection of Microbial Pathogens. Methods in Molecular Biology™, vol 216. Humana Press. https://doi.org/10.1385/1-59259-344-5:185

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  • DOI: https://doi.org/10.1385/1-59259-344-5:185

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-049-6

  • Online ISBN: 978-1-59259-344-6

  • eBook Packages: Springer Protocols

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