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Specificity and Performance of Diagnostic PCR Assays

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PCR Detection of Microbial Pathogens

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 216))

Abstract

The undisputed success of detection assays based on the polymerase chain reaction (PCR) has been largely due to its rapidity in comparison to many conventional diagnostic methods. For instance, detection and identification of mycobacteria, chlamydiae, mycoplasmas, brucellae, and other slow-growing bacteria can be accelerated from several days to a single working day when clinical samples are directly examined. Other microbial agents that are difficult to propagate outside their natural host often remain undetected by techniques relying on cultural enrichment, thus rendering PCR the only viable alternative to demonstrate their presence. Additionally, there is the enormous potential of DNA amplification assays with regard to sensitivity and specificity.

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Authors and Affiliations

  1. Division 4: Bacterial Animal Diseases and Control of Zoonoses, Federal Institute for Health Protection of Consumers and Veterinary Medicine (BgVV), Jena, Germany

    Konrad Sachse

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  1. Konrad Sachse
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Editors and Affiliations

  1. Federal Institute for Health Protection of Consumers and Veterinary Medicine (BgVV), Jena, Germany

    Konrad Sachse (Dr. Rer. Nat.) (Dr. Rer. Nat.)

  2. University of Bern, Bern, Switzerland

    Joachim Frey PhD

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© 2003 Humana Press Inc., Totowa, NJ

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Sachse, K. (2003). Specificity and Performance of Diagnostic PCR Assays. In: Sachse, K., Frey, J. (eds) PCR Detection of Microbial Pathogens. Methods in Molecular Biology™, vol 216. Humana Press. https://doi.org/10.1385/1-59259-344-5:03

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  • DOI: https://doi.org/10.1385/1-59259-344-5:03

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-049-6

  • Online ISBN: 978-1-59259-344-6

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