PCR-Derived Methods for the Identification of Trichinella Parasites from Animal and Human Samples

  • Edoardo Pozio
  • Giuseppe La Rosa
Part of the Methods in Molecular Biology™ book series (MIMB, volume 216)

Abstract

Trichinella worms (family: Trichinellidae; phylum: Nematoda) are parasites that mainly infect mammals, including humans, although they have been found in birds and, recently, in African crocodiles (1,2). The main reservoir is represented by carnivores with cannibalistic and scavenger behavior. These parasites are widespread on all continents but Antarctica, from frigid to torrid zones. The main distinguishing feature of their life cycle is that two generations occur in the same host. The first generation (from L1 larva to adult) is present in the gut and the second generation (from a newborn larva of 80 μm in length to an infective larva of 0.6-1.0 mm in length) is present in the cell of striated muscles that is modified by the larva (referred to as the nurse cell) (3,4).

Keywords

Formalin 2H2O Ethyl EDTA Shipping 

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Copyright information

© Humana Press Inc., Totowa, NJ 2003

Authors and Affiliations

  • Edoardo Pozio
    • 1
  • Giuseppe La Rosa
    • 1
  1. 1.Laboratory of ParasitologyIstituto Superiore di SanitàRomeItaly

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