Detection and Subtyping of ShigaToxin-Producing Escherichia coli (STEC)

  • Peter Gallien
Part of the Methods in Molecular Biology™ book series (MIMB, volume 216)


When the Japanese microbiologist Shiga discovered a bacterium causing dysentery in humans in 1898, the organism was designated Shigella dysenteriae type 1. The toxin produced by the germ was found to have enterotoxic and neurotoxic properties. Later on, it became clear that, in most countries, Shigella dysenteriae type 1 does not play an important role in human infectious diseases. Nevertheless, this microorganism has spread a genetic message among other bacterial species, namely Escherichia coli and Citrobacter freundii.


Agar Sodium Chloride Electrophoresis MgCl2 Serin 


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Copyright information

© Humana Press Inc., Totowa, NJ 2003

Authors and Affiliations

  • Peter Gallien
    • 1
  1. 1.National Veterinary Reference Laboratory for E. ColiFederal Institute for Health Protection of Consumers and Veterinary Medicine (BgVV)DessauGermany

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