Abstract
Capillary electrophoresis (CE) was developed as a high sensitivity, high resolution, quantitative, electrophoretic separation technique. Since its commercial introduction in 1987, it has proved to be a versatile analytical tool for the separation of both small molecules, e.g., inorganic cations and anions, and drugs; and large molecules, e.g., peptides, proteins, carbohydrates, and nucleic acids.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
McCormick, R. M. (1994) Capillary zone electrophoresis of peptides, in Handbook of Capillary Electrophoresis (Landers, J. P., ed.), CRC, Boca Raton, FL, 287–324.
Strickland, M. and Strickland, N. (1990) Free solution capillary electrophoresis using phosphate buffer and acidic pH. Am. Lab. 22, 60–65.
Chen, F.A., Kelly, L., Palmieri, R., Biehler, R., and Schwartz, H. (1992) Use of high ionic strength buffers for the separation of proteins and peptides with CE. J. Liq. Chrom. 15, 1143–1150.
Burgi, D. S. and Chien, R. L. (1992) On-column sample concentration using field-amplification in CZE. Anal. Chem. 64, 849–855A.
Kenny, J. W., Ohms, J. I., and Smith, A. J. (1993) Micropreparative capillary electrophoresis (MPCE) and micropreparative HPLC of protein digests, in Techniques in Protein Chemistry IV (Hogue Angeletti, R.A., ed.), Academic, San Diego, CA, pp. 363–370.
Bergman, T. and Jornvall, H. (1992) Capillary electrophoresis for preparation of peptides and direct determination of amino acids, in Techniques in Protein Chemistry III (Hogue Angeletti, R. A., ed.), Academic, San Deigo, CA, pp. 129–134.
Righetti, P. G., Gelfi, C., Perego, M., Stoyanov, A. V., and Bossi, A. (1997) Capillary zone electrophoresis of oligonucleotides and peptides in isoelectric buffers: theory and Methodology. Electrophoresis 18, 2143–2153
Palmer, J., Burgi, D. S., Munro, N. J., and Landers, J. P. (2001) Electrokinetic injection for stacking neutral analytes in capillary and microchip electrophoresis. Anal. Chem. 73, 725–731.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2003 Humana Press Inc.
About this protocol
Cite this protocol
Smith, A.J. (2003). Analytical and Micropreparative Capillary Electrophoresis of Peptides. In: Smith, B.J. (eds) Protein Sequencing Protocols. Methods in Molecular Biology™, vol 211. Humana Press. https://doi.org/10.1385/1-59259-342-9:91
Download citation
DOI: https://doi.org/10.1385/1-59259-342-9:91
Publisher Name: Humana Press
Print ISBN: 978-0-89603-975-9
Online ISBN: 978-1-59259-342-2
eBook Packages: Springer Protocols