Abstract
Tandem mass spectrometry has been used for many years as a technique for sequencing peptides (1). In conjunction with liquid chromatography and database searching, this technique has developed into a very powerful means of elucidating protein identities in complex mixtures (2–5). These mixtures may result from an affinity enrichment or immuno-precipitation of an extract with a “bait” protein. Whole protein complexes may be enriched, such as the ribosome, which contains over 70 proteins (3). With recent improvements, greater mixtures of proteins have been identified. It is now possible to directly identify proteins from organelles and whole-cell extracts containing several thousand proteins using chromatography, tandem mass spectrometry, and database searching (6).
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Schieltz, D.M., Yates, J.R. (2003). Direct Identification of Proteins in Ultracomplex Mixtures. In: Smith, B.J. (eds) Protein Sequencing Protocols. Methods in Molecular Biology™, vol 211. Humana Press. https://doi.org/10.1385/1-59259-342-9:235
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DOI: https://doi.org/10.1385/1-59259-342-9:235
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