Abstract
Following the commercial introduction as "e;AAA Direct,"e; amino acid analysis by anion exchange chromatography and integrated pulsed amperometric detection (IPAD) (1) has been applied in many different types of amino acid assays. The new method does not require any derivatization. It is thus easier to use and less costly than derivatizaton-based methods. Additional savings are achieved by the relatively inexpensive single-component mobile phases and by the low flow rate (0.25 mL/min). The limits of detection are in the femtomol range and linear calibration plots extend over three orders of magnitude for the most amino acids (1).
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Jandik, P., Cheng, J., Avdalovic, N. (2003). Amino Acid Analysis in Protein Hydrolysates Using Anion Exchange Chromatography and IPAD Detection. In: Smith, B.J. (eds) Protein Sequencing Protocols. Methods in Molecular Biology™, vol 211. Humana Press. https://doi.org/10.1385/1-59259-342-9:155
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DOI: https://doi.org/10.1385/1-59259-342-9:155
Publisher Name: Humana Press
Print ISBN: 978-0-89603-975-9
Online ISBN: 978-1-59259-342-2
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