Abstract
The method described in this chapter is based on the derivatization of amino acids, produced by hydrolysis of peptides and proteins, with phenylisothiocyanate (PITC) under alkaline conditions. This forms the phenylthiocarbamyl (PTC-) amino acids, which are then separated by reverse-phase high-performance liquid chromatography (RP-HPLC) and quantified from their UV-absorbance at 254 nm.
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Brent Irvine, G., Davidson, I. (2003). Amino Acid Analysis. In: Smith, B.J. (eds) Protein Sequencing Protocols. Methods in Molecular Biology™, vol 211. Humana Press. https://doi.org/10.1385/1-59259-342-9:123
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DOI: https://doi.org/10.1385/1-59259-342-9:123
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