Abstract
High-performance liquid chromatography (HPLC) is one of the most common separation techniques in today’s protein chemistry. With the development of diode-array UV detectors for HPLC instruments on-line derivative spectroscopy has become possible and provides an extremely powerful tool for the analysis of peptides and proteins (2). The method of on-line spectral analysis has been particularly useful in the analysis of peptides containing aromatic residues (1,2). The formation of second derivatives of the absorption maxima leads to further increases in the resolution between spectral differences and allows one to distinguish between different aromatic residues in a peptide. Based on these findings we have utilized HPLC on-line derivative spectroscopy for the analysis of an important post-translational modification of peptides and proteins (1).
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Turck, C.W. (2003). High-Performance Liquid Chromatography On-Line Derivative Spectroscopy for the Characterization of Peptides with Aromatic Amino Acid Residues. In: Smith, B.J. (eds) Protein Sequencing Protocols. Methods in Molecular Biology™, vol 211. Humana Press. https://doi.org/10.1385/1-59259-342-9:101
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DOI: https://doi.org/10.1385/1-59259-342-9:101
Publisher Name: Humana Press
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