Abstract
Gene targeting in mouse embryonic stem cells is now a well-established technique that is widely used to create animal models for human disease or to study gene function at the level of the whole animal. The purpose of this chapter is to provide a detailed protocol to investigators without previous experience in gene targeting and embryonic stem (ES) cell technology. This protocol is an adaptation of previously published protocols (1,2) and has been successfully applied by investigators at the Mayo Clinic in Rochester and in several other institutions.
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References
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© 2003 Humana Press Inc.
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van Deursen, J. (2003). Gene Targeting in Mouse Embryonic Stem Cells. In: Hofker, M.H., van Deursen, J. (eds) Transgenic Mouse. Methods in Molecular Biology™, vol 209. Humana Press. https://doi.org/10.1385/1-59259-340-2:145
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DOI: https://doi.org/10.1385/1-59259-340-2:145
Publisher Name: Humana Press
Print ISBN: 978-0-89603-915-5
Online ISBN: 978-1-59259-340-8
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