Wound Healing pp 255-261 | Cite as

Suction Blister Model of Wound Healing

  • Vesa Koivukangas
  • Aarne Oikarinen
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 78)


The suction blister model was originally developed for the separation of viable epidermis from dermis by Kiistala (1). Since its development, its use has been expanded and applied to several other applications. One new application assesses the collagen synthesis rate in the human skin in vivo by using the suction blister fluid collected in an assay of collagen propeptides (2). This method is sensitive and can detect changes in collagen synthesis owing to various diseases and topical or systemic therapies. Other applications of the suction blister technique include measurements of pharmacological agents or their derivatives from interstitial fluid, and assays of various enzymes, cytokines, and so on (3-7). Additionally, the suction blister technique has even been used to treat vitiligo by collecting living melanocytes from blister roofs from healthy skin and injecting these into blister cavities induced in diseased skin.


Healthy Skin Blister Fluid Viable Epidermis Suction Blister Electric Lamp 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    Kiistala, U. (1968) Suction blister device for separating viable epidermis from dermis. J. Invest. Dermatol. 50, 129–137.PubMedGoogle Scholar
  2. 2.
    Oikarinen, A., Autio, P., Kiistala, U., Risteli, L., and Risteli, J. (1992) A new method to measure type I and III collagen synthesis in human skin in vivo: demonstration of decreased collagen synthesis after topical glucocorticoid treatment. J. Invest. Dermatol. 98, 220–225.PubMedCrossRefGoogle Scholar
  3. 3.
    Ingemansson-Nordqvist, B., Kiistala, U., and Rorsman, H. (1967) Culture of adult human epidermal cells obtained from roofs of suction blisters. Acta Dermatol. Venereol. 47, 237–240.Google Scholar
  4. 4.
    Averbeck, D., Averbeck, S., Blais, J., Moysan, A., Hüppe, G., Morliére, P., Prognon, P., Vigny, P., and Dubertret, L. (1989) Suction blister fluid: its use for pharmacodynamic and toxicological studies of drugs and metabolites in vivo in human skin after topical or systemic administration, in Models in Dermatology, vol. 4 (Maibach, H. I. and Lowe, N. J., eds.), Karger, Basel, pp. 5–11.Google Scholar
  5. 5.
    Benfeldt, E., Serup, J., and Menné, T. (1999) Microdialysis vs. suction blister technique for in vivo sampling of pharmacokinetics in the human dermis. Acta Dermatol. Venereol. 79, 338–342.CrossRefGoogle Scholar
  6. 6.
    Oikarinen, A., Kylmäniemi, M., Autio-Harmainen, H., Autio, P., and Salo, T. (1993) Demonstration of 72-kDa and 92-kDa forms of type IV collagenase in human skin: variable expression in various blistering diseases, induction during re-epithelialization and decrease by topical glucocorticoids. J. Invest. Dermatol. 101, 205–210.PubMedCrossRefGoogle Scholar
  7. 7.
    Kylmäniemi, M., Autio, P., and Oikarinen, A. (1994) Interleukin 1alfa (IL-1alfa) in human skin in vivo. Lack of correlation to markers of collagen metabolism. Acta Dermatol. Venereol. 74, 364–367.Google Scholar
  8. 8.
    Kiistala, U. and Mustakallio, K. K. (1967) Dermo-epidermal separation with suction: electron microscopic and histochemical study of initial events of blistering on human skin. J. Invest. Dermatol. 48, 466–477.PubMedGoogle Scholar
  9. 9.
    Oikarinen, A., Savolainen, E.-R., Tryggvason, K., Foidart, J.-M., and Kiistala, U. (1982) Basement membrane components and galactosylhydroxylysyl glucosyltransferase in suction blisters of human skin. Br. J. Dermatol. 106, 257–266.PubMedCrossRefGoogle Scholar
  10. 10.
    Kainulainen, T., Häkkinen, L., Hamidi, S., Larjava, K., Kallioinen, M., Peltonen J., Salo, T., Larjava, H., and Oikarinen, A. (1998) Laminin-5 expression is independent of the injury and microenvironment during re-epithelialization of wounds. J. Histochem. Cytochem. 46, 353–360.PubMedCrossRefGoogle Scholar
  11. 11.
    Leivo, T., Kiistala, U., Vesterinen, M., Owaribe, K., Burgeson, R. E., Virtanen, I., and Oikarinen, A. (2000) Re-epithelialization rate and protein expression in suction-induced wound model: comparison between intact blisters, open wounds and calcipotrial-pretreated open wounds. Br. J. Dermatol. 142, 991–1002.PubMedCrossRefGoogle Scholar
  12. 12.
    Kiistala, U. (1972) Dermal-epidermal separation II. External factors in suction blister formation with special reference to the effects of temperature. Ann. Clin. Res. 4, 236–246.PubMedGoogle Scholar
  13. 13.
    Koivukangas, V., Annala, A.-P., Salmela, P. I., and Oikarinen, A. (1999) Delayed restoration of epidermal barrier function after suction blister injury in patients with diabetes mellitus. Diabet. Med. 16, 563–567.PubMedCrossRefGoogle Scholar
  14. 14.
    Koivukangas, V. and Oikarinen, A. (1998) Effects of PUVA and UVB treatment on restoration of epidermal barrier function and vascular response after suction blister injury in human skin in vivo. Photodermatol. Photoimmunol. Photomed. 14, 119–124.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2003

Authors and Affiliations

  • Vesa Koivukangas
    • 1
  • Aarne Oikarinen
    • 1
  1. 1.Departments of Dermatology and SurgeryUniversity of OuluFinland

Personalised recommendations