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Growth Control by the Retinoblastoma Gene Family

  • Protocol
Tumor Suppressor Genes

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 222))

Abstract

The Retinoblastoma family consists of three genes, RB, p107, and Rb2/p130, all fundamental in the control of important cellular phenomena, such as cell cycle, differentiation, and apoptosis. The “founder” and the most investigated gene of the family is RB, which is considered the prototype for the tumor suppressor genes (1,2). The other two genes, p107 and Rb2/p130, and the proteins they code for, p107 and pRb2/p130, respectively, clearly reflect a high degree of structural and functional similarity to the RB gene product, pRb (3,4). The RB family proteins were disclosed initially by investigators working on viral oncoproteins. In particular, a set of proteins associated with the Adenovirus 5 E1A oncoprotein was identified, and the bands representing the most abundant ones were named p60, p105, p107, p130, and p300, in keeping with their apparent molecular mass, as determined by SDS-PAGE (5). The subsequent characterization of these proteins identified p105 as the product of the RB gene (6). Later, genes encoding p107 (7,8) and pRb2/p130 (9–11) were cloned using different strategies.

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Authors and Affiliations

  1. Center for Experimental Research, Regina Elena Cancer Institute, Rome, Italy

    Marco G. Paggi

  2. CNR, Istituto di Neurobiologia e Medicina Molecolare, Rome, Italy

    Armando Felsani

  3. Sbarro Institute for Cancer Research and Molecular Medicine, Temple University, Philadelphia, PA

    Antonio Giordano

Authors
  1. Marco G. Paggi
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  2. Armando Felsani
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  3. Antonio Giordano
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Editors and Affiliations

  1. Departments of Medicine, Genetics, and Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, PA

    Wafik S. El-Deiry MD, PhD

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© 2003 Humana Press Inc.

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Paggi, M.G., Felsani, A., Giordano, A. (2003). Growth Control by the Retinoblastoma Gene Family. In: El-Deiry, W.S. (eds) Tumor Suppressor Genes. Methods in Molecular Biology™, vol 222. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-328-3:003

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  • DOI: https://doi.org/10.1385/1-59259-328-3:003

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-986-5

  • Online ISBN: 978-1-59259-328-6

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