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Oligonucleotide Ligation Assay

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Single Nucleotide Polymorphisms

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 212))

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Abstract

The ability of DNA ligases to join nucleic acids is strongly influenced by mismatches in the ligation substrates (1–3). This mechanism has been exploited in a number of assays where the ability of oligonuleotide probes to be ligated reflects the genotype of the target molecules. This chapter will describe two protocols for solid-phase detection of reaction products in the oligonucleotide ligation assay (OLA), although there are several other detection schemes in use. However, the general considerations of ligase-based sequence distinction are the same, and they will be described in some detail.

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Author information

Authors and Affiliations

  1. Rudbeck Laboratory, Unit of Molecular Medicine, Department of Genetics and Pathology, Uppsala University, Uppsala, Sweden

    Jonas Jarvius, Mats Nilsson & Ulf Landegren

Authors
  1. Jonas Jarvius
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  2. Mats Nilsson
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  3. Ulf Landegren
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Editor information

Editors and Affiliations

  1. Cardiovascular Research Institute and Department of Dermatology, University of California, San Francisco, San Francisco, CA, USA

    Pui-Yan Kwok MD, PhD

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© 2003 Humana Press Inc.

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Jarvius, J., Nilsson, M., Landegren, U. (2003). Oligonucleotide Ligation Assay. In: Kwok, PY. (eds) Single Nucleotide Polymorphisms. Methods in Molecular Biology™, vol 212. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-327-5:215

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  • DOI: https://doi.org/10.1385/1-59259-327-5:215

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-968-1

  • Online ISBN: 978-1-59259-327-9

  • eBook Packages: Springer Protocols

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