Conformation-Sensitive Gel Electrophoresis
Several large databases are now available which contain information on hundreds of thousands of single nucleotide polymorphisms (SNPs) distributed throughout the genome. Although these databases represent a tremendous resource for studies of human variation and disease, two challenges remain. First is the development of novel strategies of genotyping known SNP based genetic markers that will allow accurate and rapid high-throughput analyses (1,2). Second is the development of sensitive and specific methods for the detection of previously unknown, novel SNPs in particular genes or genomic regions of special interest. The various methods currently available for detection of SNPs all depend on the ability to detect different physical properties in DNA molecules that result from variations in the nucleotide sequence. These properties include minor differences in thermal melting profiles of two DNA molecules differing in sequence by a single base or structural distortions in perfectly double stranded nucleic acid molecules due to the presence of unpaired or mismatched bases.
KeywordsEthidium Bromide Solution Differential Migration Heteroduplex Formation Acrylamide Solution Single Base Difference
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