Abstract
Different methods for delivery of genes to the lungs such as intravenous injection, and nasal, or intratracheal instillation have been reported in animal models. However, these strategies are invasive or they may not distribute the material uniformly throughout pulmonary tissues. Currently, the technique used for administering gene delivery vectors to the lungs is mainly through injections by the use of computed tomography-guided percutaneous fine-needle injections or bronchoscopy (1,2). This procedure is very invasive and targets a very restricted region in the lungs.
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References
Swisher, S. G., Roth, J. A., Nemunaitis, J., et al. (1999) Adenovirus-mediated p53 gene transfer in advanced non-small-cell lung cancer. J. Natl. Cancer Inst. 91(9), 763–771.
Zuckerman, J. B., Robinson, C. B., McCoy, K. S., et al. (1999) A phase I study of adenovirus-mediated transfer of the human cystic fibrosis transmembrane conductance regulator gene to a lung segment of individuals with cystic fibrosis. Hum. Gene Ther. 10(18), 2973–2985.
Stribling, R., Brunette, E., Liggit, D., Gaensler, K., and Debs, R. (1992) Aerosol gene delivery in vivo. Proc. Natl. Acad. Sci. USA 89, 11277–11281.
Eastman, S. J., Lukason, M. J., Tousignant, J. D., Murray, H., Lane, M. D., St. George, J. A., et al. (1997) A concentrated and stable aerosol formulation of cationic lipid:DNA complexes giving high level gene expression in mouse lung. Hum. Gene Ther. 8, 765–773.
McDonald, R. J., Liggitt, H. D., Roche, L., Nguyen, H. T., Pearlman, R., Raabe, O. G., et al. (1998) Aerosol delivery of lipid:DNA complexes to lungs of rhesus monkeys. Pharm. Res. 15(5), 671–679.
Gonda, I. (1992) in Pharmaceutical Inhalation Aerosol Technology (Hickey, A. J., ed.), Marcel Dekker, New York, pp. 61–82.
Koshkina, N. V., Knight, V., Gilbert, B. E., Golunski, E., Roberts, L., and Waldrep, J. C. (2001) Improved respiratory delivery of anticancer drugs, camptothecin and paclitaxel, with 5% CO2 enriched air: Pharmacokinetic studies. Cancer Chemother. Pharmacol. 47, 451–456.
Densmore, C. L., Orson, F. M., Xu, B., et al. (2000) Aerosol delivery of robust polyethyleneimine-DNA complexes for gene therapy and genetic immunization. Mol. Ther. 1(2), 180–188.
Gautam, A., Densmore, C. L., Xu, B., and Waldrep, J. C. (2000) Enhanced gene expression in mouse lung after PEI-DNA aerosol delivery. Mol. Ther. 2(1), 63–70.
Gautam, A., Densmore, C. L., and Waldrep, J. C. (2000) Inhibition of experimental lung metastasis by aerosol delivery of PEI-p53 complexes. Mol. Ther. 2(4), 318–323.
Densmore, C. L., Kleinerman, E. S., Gautam, A., et al. (2001) Growth suppression of established human osteosarcoma lung metastases in mice by aerosol gene therapy with PEI:p53 complexes. Cancer Gene Therapy 8(9), 619–627.
Gautam, A., Densmore, C. L., and Waldrep, J. C. Pulmonary cytokine responses associated with PEI-DNA aerosol gene therapy. Gene Ther. 8, 254–257.
Scheule, R. K., et al. (1997) Basis of pulmonary toxicity associated with cationic lipid-mediated gene transfer to the mammalian lung. Hum. Gene Ther. 8(6), 689–707.
Kafri, T., Morgan, D., Krahl, T., Sarvetnick, N., Sherman, L., and Verma, I. (1998) Cellular immune response to adenoviral vector infected cells does not require de novo viral gene expression: implications for gene therapy. Proc. Natl. Acad. Sci. USA 95(19), 11377–11382.
Gautam, A., Densmore, C. L., Golunski, E., Xu, B., and Waldrep, J. C. (2001) Transgene expression in mouse airway epithelium by aerosol gene therapy with PEI-DNA complexes. Mol. Ther. 3(4), 551–556.
Knight, V., Koshkina, N. V., Waldrep, J. C., Giovanella, B. C., and Gilbert, B. E. (1999) Anticancer effect of 9-Nitrocamptothecin liposome aerosol on human cancer xenografts in nude mice. Cancer Chemother. Pharmacol. 44, 177–186.
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© 2003 Humana Press Inc., Totowa, NJ
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Gautam, A., Waldrep, J.C., Orson, F.M., Kinsey, B.M., Xu, B., Densmore, C.L. (2003). Topical Gene Therapy for Pulmonary Diseases with PEI-DNA Aerosol Complexes. In: Driscoll, B. (eds) Lung Cancer. Methods in Molecular Medicine™, vol 75. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-324-0:561
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DOI: https://doi.org/10.1385/1-59259-324-0:561
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-920-9
Online ISBN: 978-1-59259-324-8
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