Abstract
Lung cancer is the most common fatal type of cancer in the developed world. The overwhelming majority of cases of lung cancer are caused by tobacco products (1), and even with the best therapeutic approaches, less than 15% of diagnosed cases survive 5 years (2). It has been noted that even after smoking cessation, the risk of lung cancer remains elevated for over 15 years (3). This observation clearly indicates that lung cancer has a protracted course developing over a 10-20-yr period, from the moment in which an epithelial cell becomes initiated by chronic exposure to chemical insults until a clinically evident cancer is detected. The long latency period generated by this biology provides an important window of opportunity to find transformed precancerous cells in high-risk populations and intervene in a timely manner.
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References
Peto, R., López, A. D., Boreham, J., Thun, M., and Heath, C. (1992) Mortality from tobacco in developed countries: indirect estimation from national vital statistics. Lancet 339, 1268–1278.
Mulshine, J. L. (1998) Lung cancer: current progress, future promise. Primary Care Cancer 18, 9–12.
Gaffney, M. and Altshuler, B. (1988) Examination of the role of cigarette smoke in lung carcinogenesis. J. Natl. Cancer Inst. 80, 925–931.
Aunoble, B., Sanches, R., Didier, E., and Bignon, Y. (2000) Major oncogenes and tumor suppressor genes involved in epithelial ovarian cancer. Int. J. Oncol. 16, 567–576.
Sakorafas, G. H., Tsiotou, A. G., and Tsiotos, G. G. (2000) Molecular biology of pancreatic cancer; oncogenes, tumor suppressor genes, growth factors, and their receptors from a clinical perspective. Cancer Treat. Rev. 26, 29–52.
Minamoto, T., Mai, M., and Ronai, Z. (2000) K-ras mutation: early detection of molecular diagnosis and risk assessment of colorectal, pancreas, and lung cancers. Cancer Detect. Prev. 24, 1–12.
Slebos, R. J. and Rodenhuis, S. (1992) The ras gene family in human non-small-cell lung cancer. Monogr. Natl. Cancer Inst. 13, 23–29.
Suzuki, Y., Orita, M., Shiraishi, M., Hayashi, K., and Sekiya, T. (1990) Detection of ras gene mutations in human lung cancer by single-strand conformation polymorphism analysis of polymerase chain reaction products. Oncogene 5, 1037–1043.
Kumar, R. and Dunn, L. L. (1989) Designed diagnostic restriction fragment length polymorphisms for the detection of point mutations in ras oncogenes. Oncogene Res. 1, 235–241.
Jacobson, D. R. and Mills, N. E. (1994) A highly sensitive assay for mutant ras genes and its application to the study of presentation and relapse genotypes in acute leukemia. Oncogene 9, 553–563.
Lehman, T. A., Scott, F., Seddon, M., Kelly, K., Dempsey, E. C., Wilson, V. L., et al. (1996) Detection of K-ras oncogene mutations by polymerase chain reactionbased ligase chain reaction. Anal. Biochem. 239, 153–159.
Scott, F., Modali, R., Lehman, T. A., Seddon, M., Kelly, K., Dempsey, E. C., et al. (1997) High frequency of K-ras codon 12 mutations in bronchoalveolar lavage fluid of patients at high risk for second primary lung cancer. Clin. Cancer Res. 3, 479–482.
Scott, F., Cuttitta, F., Treston, A. M., Avis, I., Gupta, P., Ruckdeschel, J., et al. (1993) Prospective trial evaluating immunocytochemical-based sputum techniques for early lung cancer detection: assays for promotion factors in the bronchial lavage. J. Cell. Biochem. Suppl. 17F, 175–183.
Wiedmann, M., Wilson, W. J., Czajka, J., Luo, J., Barany, F., and Batt, C. A. (1994) Ligase chain reaction (LCR): overview and applications. PCR Methods Appl. 3 S51–S64.
Mitsudomi, T., Viallet, J., Mulshine, J. L., Linnoila, R. I., Minna, J. D., and Gazdar, A. E (1991) Mutations of ras genes distinguish a subset of non-small-cell lung cancer cell lines from small-cell lung cancer cell lines. Oncogene 6, 1353–1362.
Livak, K. J., Flood, S. J., Marmaro, J., Giusti, W., and Deetz, K. (1995) Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR products and nucleic acid hybridization. PCR Methods Appl. 4, 357–362.
Finney, R. E. and Bishop, J. M. (1993) Predisposition to neoplastic transformation caused by gene replacement of H-ras 1. Science 260, 1524–1527.
Parada, L. F., Land, H., Weinberg, R. A., Wolf, D., and Rotter, V. (1984) Cooperation between gene encoding p53 tumour antigen and ras in cellular transformation. Nature 312, 649–651.
Sidransky, D. (1994) Molecular screening; how long can we afford to wait? J. Natl. Cancer Inst. 87, 955–956.
Tockman, M. S., Gupta, P. K., Myers, J. D., Frost, J. K., Baylin, S. B., Gold, E. B., et al. (1988) Sensitive and specific monoclonal antibody recognition of human lung cancer antigen on preserved sputum cells: A new approach to early lung cancer detection. J. Clin. Oncol. 6, 1685–1693.
Tockman, M. S., Mulshine, J. L., Piantadosi, S., Erozan, Y. S., Gupta, P. K., Ruckdeschel, J. C., et al. (1997) Prospective detection of preclinical lung cancer: results from two studies of hnRNP expression. Clin. Cancer Res. 3, 2237–2246.
Fielding, P. Turnbull, L., Prime, W., Walshaw, M., and Field, J. K. (1999) Heterogeneous nuclear ribonucleoprotein A2/B1 up-regulation in bronchial lavage specimens: a clinical marker of early lung cancer detection. Clin. Cancer Res. 5, 4048–4052.
Mulshine, J. L. and Henschke, C. I. (2000) Prospects for lung-cancer screening. Lancet 355, 592–593.
Wattenberg, L. W., Wiedmann, T. S., Estensen, R. D., Zimmerman, C. L., Galbraith, A. R., Steele, V. E., and Kelloff, G. J. (2000) Chemoprevention of pulmonary carcinogenesis by brief exposures to aerosolized budesonide or beclomethasone dipropionate and by the combination of aerosolized budesonide and dietary myoinositol. Carcinogenesis 21, 179–182.
Dahl, A. R., Grossi, I. M., Houchens, D. P., Scovell, L. J., Placke, M. E., Imondi, A. R., et al. (2000) Inhaled isotretinoin (13-cis retinoic acid) is an effective lung cancer chemopreventive agent in A/J mice at low doses: a pilot study. Clin. Cancer Res. 8, 3015–3024.
Wang, D. L., Marko, M., Dahl, A. R., Engelke, K. S., Placke, M. E., Imondi, A. R., et al. (2000) Topical delivery of 13-cis retinoic acid by inhalation up-regulates expression of rodent lung but not liver retinoic acid receptors. Clin. Cancer Res. 9, 3636–3645.
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© 2003 Humana Press Inc., Totowa, NJ
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MartÍnez, A., Lehman, T.A., Modali, R., Mulshine, J.L. (2003). Screening of Mutations in the ras Family of Oncogenes by Polymerase Chain Reaction-Based Ligase Chain Reaction. In: Driscoll, B. (eds) Lung Cancer. Methods in Molecular Medicine™, vol 74. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-323-2:187
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DOI: https://doi.org/10.1385/1-59259-323-2:187
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-985-8
Online ISBN: 978-1-59259-323-1
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