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Haemophilus influenzae Protocols pp 93–99Cite as

Characterization of Plasmids

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Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 71))

Abstract

Antibiotic resistant strains of Haemophilus influenzae appeared in the early 1970s and studies on these strains led to the detection of large 40–50 kb conjugative plasmids (1–3). Early work was hampered by the failure to easily detect extrachromosomal plasmid in clinical isolates. However, closed circular plasmid was readily detected in exconjugants following conjugal transfer of plasmid (4). Early, it was recognized that small, approx 5-kb, nonconjugative plasmid was associated with a proportion of strains exhibiting β-lactamasepositive ampicillin resistance (1,2). We concentrate here on characterization of large conjugative plasmids.

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Author information

Authors and Affiliations

  1. Medical School of Alexandroupolis, University of Thrace, Alexandroupolis, Greece

    Ioanna D. Dimopoulou

  2. Nuffield Department of Clinical Laboratory Sciences, John Radcliffe Hospital, Headington, Oxford, UK

    Mohd-Zain Zaini & Derrick W. Crook

Authors
  1. Ioanna D. Dimopoulou
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  2. Mohd-Zain Zaini
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  3. Derrick W. Crook
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Editor information

Editors and Affiliations

  1. Department of Pediatrics, John Radcliffe Hospital, Headington, Oxford, UK

    Mark A. Herbert , Derek W. Hood  & E. Richard Moxon ,  & 

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© 2003 Humana Press Inc.

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Dimopoulou, I.D., Zaini, MZ., Crook, D.W. (2003). Characterization of Plasmids. In: Herbert, M.A., Hood, D.W., Moxon, E.R. (eds) Haemophilus influenzae Protocols. Methods in Molecular Medicine™, vol 71. Humana Press. https://doi.org/10.1385/1-59259-321-6:93

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  • DOI: https://doi.org/10.1385/1-59259-321-6:93

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-928-5

  • Online ISBN: 978-1-59259-321-7

  • eBook Packages: Springer Protocols

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