Abstract
The two animal models mainly used for the study of Haemophilus influenzae infections are the 5-d-old infant rat, for invasive disease, and the chinchilla, for local infection. We describe the 5-d-old infant rat model here and give references for the reader for the chinchilla model.
References
Moxon E. R. (1975) An animal model for experimental meningitis in infant rats, in New Approaches for Inducing Natural Immunity to Pyogenic Organisms (Robbins J. B., ed.), National Institutes of Health, Bethesda, MD, 76ā80.
Moxon E. R., Glode M. P, Sutton A., and Robbins J. B. (1977) The infant rat as a model of bacterial meningitis. J. Infect. Dis. 136(Suppl.), S186āS190.
Moxon E. R., Zwahlen A., Rubin L. G., Hoiseth S., and Connelly C. (1984) Pathogenesis of meningitis: experimental studies on the molecular basis of Haemophilus influenzae infection. Infection 12(Suppl. 1), S23āS28.
Moxon E. R. and Vaughn K. A. (1981) The type b capsular polysaccharide as a virulence determinant of Haemophilus influenzae: studies using clinical isolates and laboratory transformants. J. Infect. Dis. 143(4), 517ā524.
Moxon E. R. (1982) Experimental studies of Haemophilus influenzae infection in a rat model, in Haemophilus influenzae (Sell S. H. and Wright P. F., eds.), Elsevier, New York, 59ā71.
Smith A. L., Smith D. H., Averill D. M., Marino J., and Moxon E. R. (1973) Production of Haemophilus influenzae b meningitis in infant rats by intraperitoneal inoculation. Infect. Immun. 8, 278ā290.
Zwahlen A., Kroll J. S., Rubin L. G., and Moxon E. R. (1989) The molecular basis of pathogenicity in Haemophilus influenzae: comparative virulence of genetically-related capsular transformants and correlation with changes at the capsulation locus cap. Microb. Patho. 7(3), 225ā235.
Rubin L. G., Gloster E. S., and Carlone G. M. (1989) An infant rat model of bacteremia with Brazilian purpuric fever isolates of Hemophilus influenzae biogroup aegyptius. Brazilian Purpuric Fever Study Group. J. Infect. Dis. 160(3), 476ā482.
Nizet V., Colina K. F., Almquist J. R., Rubens C. E., and Smith A. L. (1996) A virulent nonencapsulated Haemophilus influenzae (published erratum appears in J. Infect. Dis. 1998 Jul;178 +, 296). J. Infect. Dis. 173(1), 180ā186.
Hood D. W., Deadman M. E., Allen T., et al. (1996) Use of the complete genome sequence information of Haemophilus influenzae strain Rd to investigate lipopolysaccharide biosynthesis. Mol. Microbiol. 22(5), 951ā965.
DāMello R. A., Langford P. R., and Kroll J. S. (1997) Role of bacterial Mncofactored superoxide dismutase in oxidative stress responses, nasopharyngeal colonization, and sustained bacteremia caused by Haemophilus influenzae type b. Infect. Immun. 65(7), 2700ā2706.
Bishai W. R., Howard N. S., Winkelstein J. A., and Smith H. O. (1994a) Characterization and virulence analysis of catalase mutants of Haemophilus influenzae. Infect. Immun. 62, 4855ā4860.
Moxon E. R., Smith A. L., Averill D. R., and Smith D. H. (1974) Haemophilus influenzae meningitis in infant rats after intranasal inoculation. J. Infect. Dis. 129, 154ā162.
Moxon E. R. and Murphy P. A. (1978) Haemophilus influenzae bacteremia and meningitis resulting from survival of a single organism. Proc. Natl. Acad. Sci. USA 75(3), 1534ā1536.
Halvorson H. O. (1935) The effect of chance on the mortality of experimentally infected animals. J. Bacteriol. 30, 330ā359.
Rubin L. G. (1987) Bacterial colonization and infection resulting from multiplication of a single organism. Rev. Infect. Dis. 9, 488ā493.
Meynell G. G. (1957) The applicability of the Hypothesis of Independent Action to fatal infections in mice given Salmonella typhimurium by mouth. J. Gen. Microbiol. 16, 396ā404.
Meynell G. G. and Stocker B. A. D. (1957) Some hypotheses on the aetiology of fatal infections in partially resistant hosts and their application to mice challenged with Salmonella paratyphi-B of Salmonella typhimurium by intraperitoneal injection. J. Gen. Microbiol. 16, 38ā58.
Armitage P., Meynell G. G., and Williams T. (1965) Birth-Death and other models for microbial infection. Nature 207, 570ā572.
Williams T. and Meynell G. G. (1967) Time-dependence and count-dependence in microbial infection. Nature 214, 473ā475.
TF dM. Animal models for nontypeable H. influenzae otitis media. Pediatr. Infect. Dis. 8, S40āS42.
de Maria T. F. A. M., Nichols W. A., and Leake E. R. Evaluation of the virulence of nontypeable Haemophilus influenzae lipooligosaccharide htrB and rfaD mutants in a Chinchilla model of otitis media. Infect. Immun. 64, 4431ā4435.
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Virji, M., Hill, D.J. (2003). Animal Models. In: Herbert, M.A., Hood, D.W., Moxon, E.R. (eds) Haemophilus influenzae Protocols. Methods in Molecular Medicineā¢, vol 71. Humana Press. https://doi.org/10.1385/1-59259-321-6:315
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DOI: https://doi.org/10.1385/1-59259-321-6:315
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