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Haemophilus influenzae Protocols pp 277–295Cite as

In Vitro Models of Infection I—Human Respiratory Tissue Organ Culture

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Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 71))

Abstract

The interaction of bacteria with mucosal surfaces of the respiratory tract is thought to be critical in their pathogenesis. Bacteria must overcome local defenses that include physical barriers such as mucus, beating cilia, and an intact epithelial surface; antibacterial substances such as defensins, secretory immunoglobulin A, and lysozyme; and resident phagocytes. Organ cultures can be used to study the initial interaction of bacteria with the mucosal surface and subsequent events, in particular the effects of bacteria on the structure and function of the respiratory epithelium. Organ cultures have a near-normal ratio of differentiated cell types that maintain their three-dimensional relationships with the extracellular matrix and submucosal tissues, and by incorporating an air interface they closely simulate in vitro the physiological conditions of the respiratory mucosa found in vivo.

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Author information

Authors and Affiliations

  1. Department of Microbiology, Royal Brompton Hospital and Host Defense Unit, Imperial College School of Science, Technology and Medicine, National Heart and Lung Institute, London, UK

    Andrew M. Middleton

  2. Host Defense Unit, Imperial College School of Science, Technology and Medicine, National Heart and Lung Institute, London, UK

    Paula Keig & Robert Wilson

Authors
  1. Andrew M. Middleton
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  2. Paula Keig
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  3. Robert Wilson
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Editor information

Editors and Affiliations

  1. Department of Pediatrics, John Radcliffe Hospital, Headington, Oxford, UK

    Mark A. Herbert , Derek W. Hood  & E. Richard Moxon ,  & 

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© 2003 Humana Press Inc.

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Middleton, A.M., Keig, P., Wilson, R. (2003). In Vitro Models of Infection I—Human Respiratory Tissue Organ Culture. In: Herbert, M.A., Hood, D.W., Moxon, E.R. (eds) Haemophilus influenzae Protocols. Methods in Molecular Medicine™, vol 71. Humana Press. https://doi.org/10.1385/1-59259-321-6:277

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  • DOI: https://doi.org/10.1385/1-59259-321-6:277

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-928-5

  • Online ISBN: 978-1-59259-321-7

  • eBook Packages: Springer Protocols

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