Skip to main content
SpringerLink
Log in

Cellular Microbiology of STEC Infections

An Overview

  • Protocol
E. coli

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 73))

  • 834 Accesses

Abstract

Cellular microbiology defines an emerging discipline that brings together the study of pathogenic microbes with eurkaryotic cell biology in order to investigate in detail the complex interactions that occur between pathogen and host during the process of disease. Over the years, we have seen the study of “cellular microbiology” move from research that was largely observational to, more recently, where microbes have become powerful tools to probe the complex molecular workings of the eukaryotic host cell (1). Examination of molecular mechanisms that characterize the interplay between bacteria and host cell has led to a new appreciation of microbial pathogenesis. A recurring theme that has emerged is that microorganisms have developed sophisticated mechanisms to subvert host cell signaling pathways in order to create a favorable environment for their own survival.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Cossart P., Boquet P., Normark S., and Rappuoli R. (1996) Cellular microbiology emerging. Science 271, 315–316.

    Article  CAS  PubMed  Google Scholar 

  2. Endo Y., Tsurugi K., Yutsudo T., Takeda Y., Ogasawara T., and Igarashi K. (1988) Site of action of a Vero toxin (VT2) from Escherichia coli O157:H7 and of Shiga toxin on eukaryotic ribosomes. RNA N-glycosidase activity of the toxins. Eur. J. Biochem. 171, 45–50.

    Article  CAS  PubMed  Google Scholar 

  3. Schmidt H. and Karch H. (1996) Enterohemolytic phenotypes and genotypes of shiga toxin-producing Escherichia coli O111 strains from patients with diarrhea and hemolytic-uremic syndrome. J. Clin. Microbiol. 34, 2364–2367.

    CAS  PubMed  Google Scholar 

  4. Schmidt H., Maier E., Karch H., and Benz R. (1996) Pore-forming properties of the plasmid-encoded hemolysin of enterohemorrhagic Escherichia coli O157:H7. Eur. J. Biochem. 241, 594–601.

    Article  CAS  PubMed  Google Scholar 

  5. Brunder W., Schmidt H., and Karch H. (1997) EspP, a novel extracellular serine protease of enterohaemorrhagic Escherichia coli O157:H7 cleaves human coagulation factor V. Mol. Microbiol. 24, 767–778.

    Article  CAS  PubMed  Google Scholar 

  6. Djafari S., Ebel F., Deibel C., Krämer S., Hudel M., and Chakraborty T. (1997) Characterization of an exported protease from Shiga toxin-producing Escherichia coli. Mol. Microbiol. 25, 771–784.

    Article  CAS  PubMed  Google Scholar 

  7. Burland V., Shao Y., Perna N.T., Plunkett G., Sofia H.J., Blattner F.R. (1998) The complete DNA sequence and analysis of the large virulence plasmid of Escherichia coli O157:H7. Nucleic Acids Res. 26, 4196–4204.

    Article  CAS  PubMed  Google Scholar 

  8. Brunder W., Schmidt H., and Karch H. (1996) KatP, a novel catalase-peroxidase encoded by the large plasmid of enterohaemorrhagic Escherichia coli O157:H7. Microbiology 142, 3305–3315.

    Article  CAS  PubMed  Google Scholar 

  9. Schmidt H., Henkel B., and Karch H. (1997) A gene cluster closely related to type II secretion pathway operons of gram-negative bacteria is located on the large plasmid of enterohemorrhagic Escherichia coli O157 strains. FEMS Microbiol. Lett. 148, 265–272.

    Article  CAS  PubMed  Google Scholar 

  10. Frankel G., Phillips A. D., Rosenshine I., Dougan G., Kaper J. B., and Knutton S. (1998) Enteropathogenic and enterohaemorrhagic Escherichia coli: more subversive elements. Mol. Microbiol. 30, 911–921.

    Article  CAS  PubMed  Google Scholar 

  11. Perna N. T., Mayhew G. F., Posfai G., Elliott S., Donnenberg M. S., Kaper J.B., et al. (1998) Molecular evolution of a pathogenicity island from enterohemorrhagic Escherichia coli O157:H7. Infect. Immun. 66, 3810–3817.

    CAS  PubMed  Google Scholar 

  12. Cornelis G. R. and Van Gijsegem F. (2000) Assembly and function of type III secretory systems. Annu. Rev. Microbiol. 54, 735–774.

    Article  CAS  PubMed  Google Scholar 

  13. Moon H. W., Whipp S. C., Argenzio R. A., Levine M. M., Giannella R. A. (1983) Attacking and effacing activities of rabbit and human enteropathogenic Escherichia coli in pig and rabbit intestines. Infect. Immun. 41, 1340–1351.

    CAS  PubMed  Google Scholar 

  14. Ebel F., Podzadel T., Rohde M., Kresse A. U., Krämer S., Deibel C., et al. (1998) Initial binding of Shiga toxin-producing Escherichia coli to host cells and subsequent induction of actin rearrangements depend on filamentous EspA-containing surface appendages. Mol. Microbiol. 30, 147–161.

    Article  CAS  PubMed  Google Scholar 

  15. Goosney D. L., Celli J., Kenny B., and Finlay B. B. (1999) Enteropathogenic Escherichia coli inhibits phagocytosis. Infect. Immun. 67, 490–495.

    CAS  PubMed  Google Scholar 

  16. Celli J., Olivier M., and Finlay B. B. (2001) Enteropathogenic Escherichia coli mediates antiphagocytosis through the inhibition of PI 3-kinase-dependent pathways. EMBO J. 20, 1245–1258.

    Article  CAS  PubMed  Google Scholar 

  17. Elliott S. J., Yu J., and Kaper J. B. (1999) The cloned locus of enterocyte effacement from enterohemorrhagic Escherichia coli O157:H7 is unable to confer the attaching and effacing phenotype upon E. coli K-12. Infect. Immun. 67, 4260–4263.

    CAS  PubMed  Google Scholar 

  18. Elliott S. J., Sperandio V., Giron J. A., Shin S., Mellies J. L., Wainwright L., et al. (2000) The locus of enterocyte effacement (LEE)-encoded regulator controls expression of both LEE-and non-LEE-encoded virulence factors in enteropathogenic and enterohemorrhagic Escherichia coli. Infect. Immun. 68, 6115–6126.

    Article  CAS  PubMed  Google Scholar 

  19. Knutton S., Rosenshine I., Pallen M. J., Nisan I., Neves B. C., Bain C., et al. (1998) A novel EspA-associated surface organelle of enteropathogenic Escherichia coli involved in protein translocation into epithelial cells. EMBO J. 17, 2166–2176.

    Article  CAS  PubMed  Google Scholar 

  20. Sekiya K., Ohishi M., Ogino T., Tamano K., Sasakawa C., and Abe A. (2001) Supermolecular structure of the enteropathogenic Escherichia coli type III secretion system and its direct interaction with the EspA-sheath-like structure. Proc. Natl. Acad. Sci. USA 98, 11,638–11,643.

    Article  CAS  PubMed  Google Scholar 

  21. Kresse A. U., Rohde M., and Guzman C. A. (1999) The EspD protein of enterohemorrhagic Escherichia coli is required for the formation of bacterial surface appendages and is incorporated in the cytoplasmic membranes of target cells. Infect. Immun. 67, 4834–4842.

    CAS  PubMed  Google Scholar 

  22. Wachter C., Beinke C., Mattes M., and Schmidt M. A. (1999) Insertion of EspD into epithelial target cell membranes by infecting enteropathogenic Escherichia coli. Mol. Microbiol. 31, 1695–1707.

    Article  CAS  PubMed  Google Scholar 

  23. Ide T., Laarmann S., Greune L., Schillers H., Oberleithner H., and Schmidt M.A. (2001) Characterization of translocation pores inserted into plasma membranes by type III-secreted Esp proteins of enteropathogenic Escherichia coli. Cell. Microbiol. 3, 669–679.

    Article  CAS  PubMed  Google Scholar 

  24. Wolff C., Nisan I., Hanski E., Frankel G., and Rosenshine I. (1998) Protein translocation into host epithelial cells by infecting enteropathogenic Escherichia coli. Mol. Microbiol. 28, 143–155.

    Article  CAS  PubMed  Google Scholar 

  25. Rosenshine I., Ruschkowski S., Stein M., Reinscheid D. J., Mills S. D., and Finlay B. B. (1996) A pathogenic bacterium triggers epithelial signals to form a functional bacterial receptor that mediates actin pseudopod formation. EMBO J. 15, 2613–2624.

    CAS  PubMed  Google Scholar 

  26. Kenny B., DeVinney R., Stein M., Reinscheid D. J., Frey E. A., and Finlay B. B. (1997) Enteropathogenic E. coli (EPEC) transfers its receptor for intimate adherence into mammalian cells. Cell 91, 511–520.

    Article  CAS  PubMed  Google Scholar 

  27. Gruenheid S., DeVinney R., Bladt F., Goosney D., Gelkop S., Gish G.D., et al. (2001) Enteropathogenic E. coli Tir binds Nck to initiate actin pedestal formation in host cells. Nat. Cell. Biol. 3, 856–859.

    Article  CAS  PubMed  Google Scholar 

  28. Ismaili A., McWhirter E., Handelsman M. Y., Brunton J. L., and Sherman P. M. (1998) Divergent signal transduction responses to infection with attaching and effacing Escherichia coli. Infect. Immun. 66, 1688–1696.

    CAS  PubMed  Google Scholar 

  29. Deibel C., Krämer S., Chakraborty T., and Ebel F. (1998) EspE, a novel secreted protein of attaching and effacing bacteria, is directly translocated into infected host cells, where it appears as a tyrosine-phosphorylated 90 kDa protein. Mol. Microbiol. 28, 463–474.

    Article  CAS  PubMed  Google Scholar 

  30. DeVinney R., Puente J. L., Gauthier A., Goosney D., and Finlay B. B. (2001) Enterohaemorrhagic and enteropathogenic Escherichia coli use a different Tirbased mechanism for pedestal formation. Mol. Microbiol. 41, 1445–1458.

    Article  CAS  PubMed  Google Scholar 

  31. Goosney D. L., DeVinney R., and Finlay B. B. (2001) Recruitment of cytoskeletal and signaling proteins to enteropathogenic and enterohemorrhagic Escherichia coli pedestals. Infect. Immun. 69, 3315–3322.

    Article  CAS  PubMed  Google Scholar 

  32. Phillips A. D., Giron J., Hicks S., Dougan G., and Frankel G. (2000) Intimin from enteropathogenic Escherichia coli mediates remodelling of the eukaryotic cell surface. Microbiology 146, 1333–1344.

    CAS  PubMed  Google Scholar 

  33. McNamara B. P., Koutsouris A., O’Connell C. B., Nougayrede J. P., Donnenberg M. S., and Hecht G. (2001) Translocated EspF protein from enteropathogenic Escherichia coli disrupts host intestinal barrier function. J. Clin. Invest. 107, 621–629.

    Article  CAS  PubMed  Google Scholar 

  34. Kenny B. and Jepson M. (2000) Targeting of an enteropathogenic Escherichia coli (EPEC) effector protein to host mitochondria. Cell. Microbiol. 2, 579–590.

    Article  CAS  PubMed  Google Scholar 

  35. Elliott S. J., Krejany E. O., Mellies J. L., Robins-Browne R. M., Sasakawa C., and Kaper J. B. (2001) EspG, a novel type III system-secreted protein from enteropathogenic Escherichia coli with similarities to VirA of Shigella flexneri. Infect. Immun. 69, 4027–4033.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Bakteriologie, Max-von-Pettenkofer-Institut, Munich, Germany

    Frank Ebel

  2. Groupe dImmunit&#00E9 Inn&#00E9e et Signalisation, Institut Pasteur, Paris, France

    Dana Philpott

Authors
  1. Frank Ebel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Dana Philpott
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Groupe d&'Immunité Innée et Signalisation, Institut Pasteur, Paris, France

    Dana Philpott

  2. Max-von-Pettenkofer-Institut, Bakteriologie, Munich, Germany

    Frank Ebel

Rights and permissions

Reprints and permissions

Copyright information

© 2003 Humana Press Inc.

About this protocol

Cite this protocol

Ebel, F., Philpott, D. (2003). Cellular Microbiology of STEC Infections. In: Philpott, D., Ebel, F. (eds) E. coli. Methods in Molecular Medicine™, vol 73. Humana Press. https://doi.org/10.1385/1-59259-316-X:91

Download citation

  • DOI: https://doi.org/10.1385/1-59259-316-X:91

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-939-1

  • Online ISBN: 978-1-59259-316-3

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation