Abstract
The development of retroviral gene transfer vectors has provided an effective way to deliver foreign genes into mammalian cells. This chapter will focus on the development of a simian retrovirus (SRV) gene delivery system. This includes the identification of the minimal packaging signal of a type D serogroup 2 SRV D2/RHE/OR/V1, construction of the SRV transduction and packaging vectors, generation of the SRV specific packaging cell line and production of the replication-deficient recombinant SRV.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Power, M. D., Marx, P. A., Bryant, M. L., Gardner, M. B., Barr, P. J., and Luciw, P. A. (1986) Nucleotide sequence of SRV-1, a type D simian acquired immune deficiency syndrome retrovirus. Science 231, 1567–1572.
Thayer, R. M., Power, M. D., Bryant, M. L., Gardner, M. B., Barr, P. J., and Luciw, P. A. (1987) Sequence relationships of type D retroviruses which cause simian acquired immunodeficiency syndrome. Virology 157, 317–329.
Sonigo, P., Barker, C., Hunter, E., and Wain-Hobson, S. (1986) Nucleotide sequence of Mason-Pfizer monkey virus: An immunosuppressive D-type retrovirus. Cell 45, 375–385.
Marracci, G. H., Avery, N. A., Shiigi, S. M., Couch, G., Palmer, H., Pilcher, K. Y., et al. (1999) Molecular cloning and cell-specific growth characterization of polymorphic variants of type D serogroup 2 simian retroviruses. Virology 261, 43–58.
Nandi, J. S., Bhavalkar-Potdar, V., Tikute, S., and Raut, C. G. (2000) A novel type D simian retrovirus naturally infecting the Indian Hanuman langur (Semnopithecus entellus). Virology 277, 6–13.
Bray, M., Prasad, S., Dubay, J. W., Hunter, E., Jeang, K. T., Rekosh, D., et al. (1994) A small element from the Mason-Pfizer monkey virus genome makes human immunodeficiency virus type 1 expression and replication Rev-independent. Proc. Natl. Acad. Sci. USA 91, 1256–1260.
Rizvi, T. A., Lew, K. A., Edwin, C., Murphy, J. R., and Schmidt, R. D. (1996) Role of Mason-Pfizer monkey virus (MPMV) constitutive transport element (CTE) in the propagation of MPMV vectors by genetic complementation using homologous/heterologous env genes. Virology 224, 517–532.
Rizvi, T. A., Schmidt, R. D., and Lew, K. A. (1997) Mason-Pfizer monkey virus (MPMV) constitutive transport element (CTE) functions in a position-dependent manner. Virology 236, 118–129.
Li, B., Wyman, T. E., Moudgil, T., Marracci, G. H., Ju, C. F., and Machida C. A. (1999) Nucleocytoplasmic export of type D simian retrovirus genomic RNA: identification of important genetic subregions and interacting cellular proteins. Virology 264, 37–54.
Rosenblum, L. L., Weiss, R. A., and McClure, M. O. (2000) Virus load and sequence variation in simian retrovirus type 2 infection. J. Virol. 74, 3449–3454.
Lerche, N. W., Switzer, W. M., Yee, J. L., Shanmugam, V., Rosenthal, A. N., Chapman, L. E., et al. (2000) Evidence of infection with simian type D retrovirus in persons occupationally exposed to nonhuman primates. J. Virol. 75, 1783–1789.
Morozov, V. A., Saal, F., Gessain, A., Terrinha, A., and Peries, J. (1991) Antibodies to gag gene coded polypeptides of type D retroviruses in healthy people from Guinea-Bissau. Intervirology 32, 253–257.
Morozov, V. A., Lagaye, S., Lyakh, L., and ter Meulen J. (1996) Type D retrovirus markers in healthy Africans from Guinea. Res. Virol. 147, 341–351.
Kzhyshkowska, J. G., Kiselev, A. V., Gordina, G. A., Kurmashow, V. I., Portjanko, N. M., Ostashkin, A. S., et al. (1996) Markers of type D retroviruses in children with Burkitt’s-type lymphoma. Immunol. Lett. 53, 101–104.
Ford, R. J., Donehower, L. A., and Bohannon, R. C. (1992). Studies on a type D retrovirus isolated from an AIDS patient lymphoma. AIDS Res. Hum. Retroviruses 8,742–751.
Mann, R., Mulligan, R. C. and Baltimore, D. (1983) Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus. Cell 33, 153–159.
Bender, M. A., Palmer, T. D., Gelinas, R. E., and Miller, A. D. (1987) Evidence that the packaging signal of moloney murine leukemia virus extends into the gag region. J. Virol. 61, 1639–1646.
Armentano, D., Yu, S., Kantoff, P. W., vonRuden, T., Anderson, W. F., and Gilboa, E. (1987) Effect of internal viral sequences on the utility of retroviral vectors. J. Virol. 61, 1647–1650.
Miller, A. D. and Buttimore, C. (1986) Redesign of retrovirus packaging cell lines to avoid recombination leading to helper virus production. Mol. Cell Biol. 6, 2895–2902.
Vile, R. G., Ali, M., Hunter, E., and McClure, M. O. (1992) Identification of generalised packaging sequence for D-type retroviral vector. Virology 189, 786–791.
Jeoffrey, P. H., Eric, H., and Andrew, M. L. L. (1995) Secondary structure model of the Mason-Pfizer monkey virus 5′ leader sequence: identification of a structural motif common to a variety of retroviruses. J. Virol. 69, 2175–2186.
Cone, R. D. and Mulligan, R. C. (1984) High-efficiency gene transfer into mammalian cells: Generation of helper-free recombinant retrovirus with broad mammalian host range. Proc. Natl. Acad. Sci. USA 81, 6349–6353.
Markowitz, D., Goff, S., and Bank, A. (1988) A safe packaging line for gene transfer: Separating viral genes on two different plasmids. J. Virol. 62, 1120–1124.
Rigg, R. J., Chen, J., Dando, J. S., Forestell, S. P., Plavec, I., and Bohnlein, E. (1996) A novel human amphotrophic packaging cell line: high titer, complement resistance, and improved safety. Virology 218, 290–295.
Bodine, D. M., McDonagh, K. T., Brandt, S. J., Ney, P. A., Agricola, B., Byrne, E., et al. (1990) Development of high-titer retrovirus producer cell line capable of gene transfer into rhesus monkey hematopoietic stem cells. Proc. Natl. Acad. Sci. USA 87, 3738–3742.
Naldini, L, Blomer, U., Gallay, P., Ory, D. Mulligan, R., Gage, E. H., et al. (1996) In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector. Science 272, 263–267.
Kafri, T., Van Praag, H., Ouyang, L., Gage, E. H., and Verma, I. M. (1999) A packaging cell line for lentivirus vectors. J. Virol. 73, 576–584.
Kalajzic, I., Stover, M. L., Liu, P., Kalajzic, Z., Rowe, D. W., and Lichtler, A. C. (2001) Use of VSVG-G pseudotyped retroviral vectors to target murine osteoprogenitor cells. Virology 284, 37–45.
Lee, H., Song, J. J., Kim, E., Yun, C. O., Choi, J., Lee, B., et al. (2001) Efficient gene transfer of VSV-G pseudotyped retroviral vector to human brain tumor. Gene Ther. 8, 268–273.
Ausubel, F., Brent, R., Kinston, R., Moore, D., Seidman, J., Smith, J., et al., eds. (1987) Current Protocols in Molecular Biology, Wiley, New York.
Gossen, M., Freundieb, S., Bender, G., Muller, G., Hillen, W., and Bujard, H. (1995). Transcriptional activation by tetracyclines in mammalian cells. Science 268, 1766–1769.
Li, B., Nguyen, S., Li, X., and Machida, C. A. (2001) Simian retrovirus vectors for gene transfer in nonhuman primate cells. Virus Res. 75, 155–168.
Chen, C. A. and Okayama, H. (1987) High-efficiency transformation of mammalian cells by plasmid DNA. Mol. Cell. Biol. 7, 2745–2752.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2003 Humana Press Inc.
About this protocol
Cite this protocol
Li, B., Machida, C.A. (2003). Development of Simian Retroviral Vectors for Gene Delivery. In: Machida, C.A. (eds) Viral Vectors for Gene Therapy. Methods in Molecular Medicine™, vol 76. Humana Press. https://doi.org/10.1385/1-59259-304-6:353
Download citation
DOI: https://doi.org/10.1385/1-59259-304-6:353
Publisher Name: Humana Press
Print ISBN: 978-1-58829-019-9
Online ISBN: 978-1-59259-304-0
eBook Packages: Springer Protocols