Use of Recombinant Adenovirus for Gene Transfer into the Rat Brain

Evaluation of Gene Transfer Efficiency, Toxicity, and Inflammatory and Immune Reactions
  • Andres Hurtado-Lorenzo
  • Anne David
  • Clare Thomas
  • Maria G. Castro
  • Pedro R. Lowenstein
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 76)


Adenovirus (Ad) vectors have been used to successfully deliver genes into a wide variety of non-central nervous system (CNS) tissues and organs in animal models of human disease and in several human phase I clinical trials (1, 2, 3). Adenoviruses are easily purified to the high titers required for in vivo administration and they are efficient in transducing terminally differentiated cells such as neurons and glial cells, resulting in high levels of transgene expression and spatially restricted transgene expression within the region of virus administration. To date, most vectors utilized have been first-generation partially deleted vectors. The most commonly used have been E1/E3-deleted vectors, or second- and third-generation vectors with further deletions in E2 orE4.


Brain Section Recombinant Adenovirus Stereotaxic Frame Helper Virus Gene Transfer Vector 
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Copyright information

© Humana Press Inc. 2003

Authors and Affiliations

  • Andres Hurtado-Lorenzo
    • 1
  • Anne David
    • 2
  • Clare Thomas
    • 3
  • Maria G. Castro
    • 1
  • Pedro R. Lowenstein
    • 1
  1. 1.Gene Therapeutics Research Institute, Cedars-Sinai Medical Center, and Department of MedicineUniversity of California Los Angeles (UCLA)Los Angeles
  2. 2.Molecular Medicine and Gene Therapy UnitUniversity of ManchesterManchesterUK
  3. 3.Department of Pediatrics and GeneticsStanford UniversityStanford

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