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Viral Vectors for Gene Therapy pp 113–133Cite as

Use of Recombinant Adenovirus for Gene Transfer into the Rat Brain

Evaluation of Gene Transfer Efficiency, Toxicity, and Inflammatory and Immune Reactions

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Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 76))

Abstract

Adenovirus (Ad) vectors have been used to successfully deliver genes into a wide variety of non-central nervous system (CNS) tissues and organs in animal models of human disease and in several human phase I clinical trials (1–3). Adenoviruses are easily purified to the high titers required for in vivo administration and they are efficient in transducing terminally differentiated cells such as neurons and glial cells, resulting in high levels of transgene expression and spatially restricted transgene expression within the region of virus administration. To date, most vectors utilized have been first-generation partially deleted vectors. The most commonly used have been E1/E3-deleted vectors, or second- and third-generation vectors with further deletions in E2 orE4.

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Author information

Authors and Affiliations

  1. Gene Therapeutics Research Institute, Cedars-Sinai Medical Center, and Department of Medicine, University of California Los Angeles (UCLA), Los Angeles, CA

    Andres Hurtado-Lorenzo, Maria G. Castro & Pedro R. Lowenstein

  2. Molecular Medicine and Gene Therapy Unit, University of Manchester, Manchester, UK

    Anne David

  3. Department of Pediatrics and Genetics, Stanford University, Stanford, CA

    Clare Thomas

Authors
  1. Andres Hurtado-Lorenzo
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  2. Anne David
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  3. Clare Thomas
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  4. Maria G. Castro
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  5. Pedro R. Lowenstein
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Editor information

Editors and Affiliations

  1. Department of Oral Molecular Biology, School of Dentistry Oregon Health & Science University, Portland, OR

    Curtis A. Machida

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© 2003 Humana Press Inc.

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Hurtado-Lorenzo, A., David, A., Thomas, C., Castro, M.G., Lowenstein, P.R. (2003). Use of Recombinant Adenovirus for Gene Transfer into the Rat Brain. In: Machida, C.A. (eds) Viral Vectors for Gene Therapy. Methods in Molecular Medicine™, vol 76. Humana Press. https://doi.org/10.1385/1-59259-304-6:113

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  • DOI: https://doi.org/10.1385/1-59259-304-6:113

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-019-9

  • Online ISBN: 978-1-59259-304-0

  • eBook Packages: Springer Protocols

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