High-Throughput Purification of PolyHis-Tagged Recombinant Fusion Proteins
Part of the Methods in Molecular Biology™ book series (MIMB, volume 205)
Methods for the efficient overexpression and purification of recombinant proteins are of paramount importance for biotechnology. In particular, for the era of functional genomics that we have entered after sequencing complete genomes, this has become a routine matter. High-throughput protein purification will, therefore, become a key technology to unravel the function of gene products (Fig. 1). To facilitate the procedure of protein purification, several tags to generate fusion proteins are available (e.g., polyHis, GST, MBP, CBP, and the like) for parallel purification using matrices coupled with affinity anchors, like Ni2+-nitrilotriacetic acid (Ni2+-NTA) which is a powerful chelating ligand for the purification of His6-tagged proteins under native conditions. Ni-NTA affinity matrices allow to purify the protein of interest contained in a crude protein mixture at a concentration of 1% in one step to more than 95% homogeneity (1).
KeywordsElution Buffer Protein Purification Nitrilotriacetic Acid Refrigerate Centrifuge Purify Enzyme Preparation
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
© Humana Press Inc. 2003