Dual-Expression Vectors for Efficient Protein Expression in Both E. coli and Mammalian Cells
In the near future, the nucleotide sequence of the genomes from many different organisms will be available. The next and more challenging step will be to characterize the biological role of each gene and the way in which the encoded protein functions in the cell. Dual-expression vectors for expression of proteins encoded by these genes in mammalian and bacterial cells can be used for this characterization. Typically, eukaryotic genes are expressed in mammalian cells to characterize biological functions and in bacterial cells to facilitate isolation of the protein. This generally requires the use of more than one vector. In contrast, use of a dual-expression vector eliminates the need to subclone from one vector system to another by combining the essential features of both eukaryotic and prokaryotic vectors in a single vector.
KeywordsNickel Agar Codon Electrophoresis Polypropylene
- 1.Mullinax, R. L., Davis, H. A., Wong, D. T., et al. (2000) Sequence-validated and expression-tested human cDNA in a dual expression vector. Strategies 13, 41–43.Google Scholar
- 2.Davis, H. A., Wong, D. T., Padgett, K. A., Sorge, J. A., and Mullinax, R. L. (2000) High-level dual mammalian and bacterial protein expression vector. Strategies 13, 136–137.Google Scholar
- 8.Felts, F., Rogers, B., Chen, K., Ji, H., Sorge, J., and Vaillancourt, P. (2000) Recombinant Renilla reniformis GFP displays low toxicity. Strategies 13, 85–87.Google Scholar
- 10.Sambrook, J., Fritsch, E. F., and Maniatis, T., eds. (1989), Molecular Cloning a Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.Google Scholar