Abstract
Bacterial artificial chromosomes (BACs) are ideal materials to use for the purpose of integrating DNA sequence with cytogenetic markers. They have been the major vectors used in genome sequencing. BACs are also well suited for fluorescent in situ hybridization (FISH) in that they represent a stable and easily manipulated form of cloned DNA that produces bright, well-defined signals on metaphase and interphase chromosome preparations (1). To link chromosomal position with DNA sequence throughout the human genome, we have developed an integrated BAC resource (1) by using FISH, PCR, and sequencing. The Resource contains a total of 6000 randomly mapped BAC clones, out of which, 1021 are BAC-sequence-tagged sites (STS) pairs representing 957 BACs (Fig. 1). This tool can be now used to rapidly identify genes affected by chromosomal rearrangements seen in genetic disorders and cancers. After this initial development, an international effort has assembled a collection of BAC clones that are both sequence-tagged and mapped relative to cytogenetic bands using FISH, resulting in a collection of 7600 clones (2; see Note 1).
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Chen, XN., Korenberg, J.R. (2002). BAC Resource for Molecular Cytogenetics. In: Fan, YS. (eds) Molecular Cytogenetics. Methods in Molecular Biology™, vol 204. Humana Press. https://doi.org/10.1385/1-59259-300-3:391
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DOI: https://doi.org/10.1385/1-59259-300-3:391
Publisher Name: Humana Press
Print ISBN: 978-1-58829-006-9
Online ISBN: 978-1-59259-300-2
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