Abstract
Chromosome aberrations are regularly detected in most hematologic neoplasms and in various solid tumors and are often associated with distinct morphologic and immunophenotypic features of certain clinico-pathologic entities of tumors (1–4). Detection of these chromosome abnormalitites provides the basis for a detailed diagnosis and for the genetic classification of tumors. Especially in leukemias and lymphomas, many primary chromosome aberrations are associated with the clinical course of the disease. Moreover, during tumor progression, additional chromosome aberrations occur. Therefore, cytogenetic findings are very helpful for predicting the prognosis and for choosing risk-adapted treatment strategies (5,6).
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
Rowley J. D. (1998) The critical role of chromosome translocations in human leukemias. Annu. Rev. Genet. 32, 495–519.
Rowley J. D. (1999) The role of chromosome translocations in leukemogenesis. Semin. Hematol. 36, 59–72.
Look A. T. (1997) Oncogenic transcription factors in human acute leukemias. Science 278, 1059–1064.
Heim S. and Mitelman F., eds. (1995) Cancer Cytogenetics Wiley-Liss, New York, NY
Rabbitts T. H. (1994) Chromosomal translocations in human cancer. Nature 372, 143–149.
Harris N. L., Jaffe E. S., Diebold J., et al. (1999) World Health Organization classification of neoplastic disease of the hematopoietic and lymphoid tissue: Report of the clinical advisory committee meeting-Airlie House, Virginia, Nov. 1997. J. Clin. Oncol. 17, 3835–3849.
Lichter P., Tang C. C., Call K., et al. (1990) High resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones. Science 247, 64–69.
Pinkel D., Straume T., and Gray J. W. (1986) Cytogenetic analysis using quantitative, high sensitivity, fluorescence hybridization. Proc. Natl. Acad. Sci. USA 83, 2934–2938.
Weber-Matthiesen K., Winkemann M., Muller-Hermelink A., Schlegelberger B., and Grote W. (1992) Simultaneous fluorescence immunophenotyping and interphase cytogenetics: a contribution to the characterization of tumor cells. J. Histochem. Cytochem. 40, 171–175.
Knuutila S., Alitalo R., and Ruutu T. (1993) Power of the MAC (Morphology Antibody Chromosomes) method in distinguishing reactive and clonal cells: report of a patient with acute lymphatic leukemia, eosinophilia and t(5;14). Gene Chromosom. Cancer 8, 219–223.
Knuutila S., Teerenhovi L., Larramendy M. L., et al. (1994) Cell lineage involvement of recurrent chromosomal abnormalities in hematologic neoplasms. Gene Chromosom Cancer 10, 95–102.
Zhang Y., Poetsch M., Weber-Matthiesen K., et al. (1996) Secondary acute leukaemias with 11q23 rearrangement: clinical, cytogenetic, FISH and FICTION studies. Brit. J. Haematol. 92, 673–680.
Zhang Y., Siebert R., Matthiesen P., et al. (2000) Feasibility of simultaneous fluorescence immunophenotyping and fluorescence in situ hybridization study for the detection of estrogen receptor expression and deletions of the estrogen receptor gene in breast carcinoma cell lines. Virchows Arch. 436, 271–276.
Knuutila S. (1997) Lineage specificity in hematological neoplasms. Br. J. Hematol. 96, 2–11.
Fagioli F., Cuneo A., Bardi A., et al. (1995) Heterogeneity of lineage involvement by trisomy 8 in myelodysplastic syndrome. Cancer Genet. Cytogenet. 82, 116–122.
Gerritsen W. R., Donohue J., Bauman J., et al. (1992) Clonal analysis of myelodysplastic syndrome: monosomy 7 is expressed in the myeloid lineage, but not in the lymphoid lineage as detected by fluorescent in situ hybridization. Blood 80, 217–224.
Kanfer E. J., Price C. M., Colman S. M., and Barrett A. J. (1992) Erythropoietinindependent colony growth in polycythaemia vera is not restricted to progenitor cells with trisomy of chromosome 8. Br. J. Haematol. 82, 773–774.
Kibbelaar R. E., van Kamp H., Dreef E. J., et al. (1992) Combined immunophenotyping and DNA in situ hybridization to syndromes. Blood 79, 1823–1828.
Anastasi J., Vardiman J. W., Rudinsky R., et al. (1991) Direct correlation of cytogenetic findings with cell morphology using in situ hybridization: an analysis of suspicious cells in bone marrow specimens of two patients completing therapy for acute lymphoblastic leukemia. Blood 77, 2456–2462.
El-Rifai W., Larramendy M. L., Ruutu T., and Knuutila S. (1995) Lymphoid involvement in a patient with acute myeloid leukemia: a direct phenotypic and genotypic study of single cells. Gene Chromosom. Cancer 15, 34–37.
Haase D., Feuring-Buske M., Konemann S., et al. (1995) Evidence for malignant transformation in acute myeloid leukemia at the level of early hematopoietic stem cells by cytogenetic analysis of CD34 positive subpopulations. Blood 86, 2906–2912.
Zhang Y., Wong K. F., Siebert R., et al. (1999) Chromosome aberrations are restricted to the CD56+, CD3-tumor cell population in natural killer cell lymphomas: A combined immunophenotyping and FISH study. Br. J. Haematol. 105, 737–742.
Price C. M., Kanfer E. J., Colman S. M., Westwood N., Barrett A. J., and Greaves M. F. (1992) Simultaneous genotypic and immunophenotypic analysis of interphase cells using dual-color fluorescence: a demonstration of lineage involvement in polycythemia vera. Blood 80, 1033–1038.
Jonas D., Lubbert M., Kawasaki E. S., et al. (1992) Clonal analysis of bcr-abl rearrangement in T lymphocytes from patients with chronic myelogenous leukemia. Blood 79, 1017–1023.
Clein G. P. and Flemans R. J. (1996) Involvement of the erythroid series in blastic crisis of chronic myeloid leukaemia: further evidence for the presence of Philadelphia chromosome in erythroblasts. Br. J. Haematol. 12, 754–758.
Fauser A. A., Kanz L., Bross K. J., and Lohr G. W. (1995) T cells and probably B cells arise from the malignant clone in chronic myelogenous leukemia. J. Clin. Invest. 75, 1080–1082.
Weber-Matthiesen K., Deerberg J., Poetsch M., Grote W., and Schlegelberger B. (1995) Numerical chromosome aberrations are present within the CD30-positive Hodgkin and Reed-Sternberg cells in 100% of analysed cases of Hodgkin’s disease. Blood 86, 1464–1468.
Wong K. F., Zhang Y., and Chan J. K. F. (1999) Cytogenetic abnormalities in natural killer cell lymphoma/leukemia—Is there a consistent pattern? Leukemia Lymphoma 34, 241–250.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2002 Humana Press Inc.
About this protocol
Cite this protocol
Zhang, Y., Schlegelberger, B. (2002). Simultaneous Fluorescence Immunophenotyping and FISH on Tumor Cells. In: Fan, YS. (eds) Molecular Cytogenetics. Methods in Molecular Biology™, vol 204. Humana Press. https://doi.org/10.1385/1-59259-300-3:379
Download citation
DOI: https://doi.org/10.1385/1-59259-300-3:379
Publisher Name: Humana Press
Print ISBN: 978-1-58829-006-9
Online ISBN: 978-1-59259-300-2
eBook Packages: Springer Protocols