Abstract
Originally the PNA oligomers were assembled on a Merrifield (methylbenzhydrylamine, MBHA)resin in a fritted vial, as shown in Fig.1 , using the Boc/Cbz strategy. Fmoc-chemistry was subsequently introduced and most recently the Mmt-monomers have also been used. The first syntheses were made in the above-mentioned glass reaction vial, but later protocols for use on fully automated equipment, both for Boc (ABI synthesizers)and Fmoc chemistry (Expedite, PerSeptive Biosystems), became available. Subheading 1.1. will only describe the manual, solid-phase synthesis of PNA oligomers from the commercially available Boc/Cbz monomers.
Reaction vessel.
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References
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Beck, F. (2002). Solid Phase Synthesis of PNA Oligomers. In: Nielsen, P.E. (eds) Peptide Nucleic Acids. Methods in Molecular Biology, vol 208. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-290-2:29
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DOI: https://doi.org/10.1385/1-59259-290-2:29
Publisher Name: Springer, Totowa, NJ
Print ISBN: 978-0-89603-976-6
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