Thermodynamics of PNA Interactions with DNA and RNA

  • Tommi Ratilainen
  • Bengt Nordén
Part of the Methods in Molecular Biology book series (MIMB, volume 208)


Thermodynamic properties of peptide nucleic acids (PNA) and their complexes with nucleic acids have attracted increasing attention. More detailed thermodynamic information is desired in order to understand and improve the behavior of PNAs in various contexts, e.g., in the design of polymerase chain reaction (PCR) probes and potentially for the use of PNA in therapeutics. The ultimate goal is to predict the thermodynamic properties of PNA-nucleic acid complexes of any sequence. For DNA and RNA thermodynamics, this has been achieved for relatively short (10–30 base pairs) doublestranded complexes (duplexes). These studies have yielded nearest neighbor parameters (ΔH° and ΔS°) for all possible combinations of base pairs in DNA and RNA (1), as well as for single mismatches in DNA (2).


Differential Scanning Calorimetry Thermodynamic Parameter Melting Curve Single Strand Peptide Nucleic Acid 
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Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  • Tommi Ratilainen
    • 1
  • Bengt Nordén
    • 1
  1. 1.Department of Physical ChemistryChalmers University of TechnologyGöteborgSweden

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