Local Forearm Vasoconstriction to Endothelin-1 Measured by Venous Occlusion Plethysmography In Vivo

  • Fiona E. Strachan
  • David J. Webb
Part of the Methods in Molecular Biology™ book series (MIMB, volume 206)


As a consequence of its potent vasoconstrictor and growth promoting properties, endothelin-1 (ET-1) has been implicated in the pathophysiology of diseases such as hypertension, heart failure and renal failure (1,2). This has lead to the rapid development of endothelin receptor antagonists as potential vasodilator treatments for cardiovascular disease (3), with some of these compounds currently being investigated in clinical trials (4, 5, 6). The previous chapters describe a number of in vitro and in vivo techniques employed in preclinical studies investigating the physiology and pathophysiology of the endothelin system. The current chapter describes the application of venous occlusion plethysmography and local intra-arterial infusion to assess the vascular effects of the endothelin system in healthy volunteers in vivo.


Forearm Blood Flow Venous Occlusion Plethysmography Chart Software Baseline Blood Flow Forearm Blood Flow Response 
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Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  • Fiona E. Strachan
    • 1
  • David J. Webb
    • 2
  1. 1.Scottish Poisons Information BureauLothian University Hospital NHS TrustEdinburghUK
  2. 2.Clinical Pharmacology Unit and Research CentreUniversity of Edinburgh, Western General HospitalEdinburghUK

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