Abstract
The autologous internal mammary artery and the long saphenous vein are the most frequently used conduits as bypass grafts in the management of occlusive arterial disease in both the coronary and lower limb circulations. However, significant stenosis occurs in over a third of lower limb reconstructions in the first postoperative year (1), and the patency rate for coronary bypass grafts is only 50% after 5 yr (2). The underlying pathological lesion of such stenoses is intimal hyperplasia (IH). IH is characterized by excessive smooth muscle cell migration and proliferation in the intima of the vessel wall, together with an accumulation of extracellular matrix. This in turn results in a significant loss in lumenal area with a subsequent reduced blood flow to the tissues.
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References
Mattos M. K., van Bemmelen P. S., Hodgson K. J., Ramsey D. E., Barkmeier L. D., and Sumner D. S. (1993) Does correction of stenoses identified with colour duplex scanning improve infrainguinal graft patency? J. Vasc. Surg. 17, 54–66.
Angelini G. D. and Newby A. C. (1989) The future of saphenous vein as a coronary artery bypass conduit. Eur. Heart J. 10, 273–280.
Freshney R. I. (1987) Culture of Animal Cells, 2nd ed., Alan R. Liss Inc., New York, pp. 298–305.
Strangeways T. S. P. and Fell H. B. (1926) Experimental studies on the differentiation of embryonic tissues growing in vivo and in vitro Proc. Royal Soc. London 99, 340–366.
Trowell O. A. (1959) The culture of mature organs in a synthetic medium. Exp. Cell Res. 16, 118–147.
Pederson D. C. and Bowyer D. E. (1985) Endothelial injury and healing in vitro. Studies using an organ culture system. Am. J. Pathol. 119, 264–272.
Koo E. W. Y. and Gotlieb A. I. (1989) Endothelial stimulation of intimal cell proliferation in a porcine aortic organ culture. Am. J. Pathol. 134, 497–503.
Koo E. W. Y. and Gotlieb A. I. (1991) Neointimal formation in the porcine aortic organ culture. Lab. Invest. 64, 743–753.
Barrett L. A., Mergner, W. J., and Trump B. F. (1979). Long term culture of human aortas. In Vitro 15, 957–966.
Soyombo A. A., Angelini G. D., Bryan A. J., Jasani B., and Newby A. C. (1990) Intimal proliferation in an organ culture of human saphenous vein. Am. J. Pathol. 137, 1401–1410.
Ferrell M., Fuster V., Gold H. K., and Chesebro J. H. (1992) A dilemma for the 1990’. Choosing appropriate experimental models for the prevention of restenosis. Circulation 85, 1630–1631.
Clozel M., Breu V., Gray G. A., Kalina B., Loffler B. M., Burri K., et al. (1994) Pharmacological characterisation of bosentan, a new potent orally active nonpeptide endothelin receptor antagonist. J. Pharmacol. Exp. Ther. 270, 228–235.
Ihara M., Ishikawa K., Fukuroda T., Saeki T., Funabashi K., Fukami T., et al. (1992) In vitro biological profile of a highly potent novel endothelin (ET) antagonist BQ-123 selective for the ETA receptor. J. Cardiovasc. Pharmacol. 20, 511–514.
Ishikawa K., Ihara M., Noguchi K., Mase T., Mino N., Saeki T., et al. (1994) Biochemical and pharmacological profile of a potent and selective endothelin Breceptor antagonist, BQ-788. Proc. Natl. Acad. Sci. USA 91, 4892–4896.
Sayers R. D., Jones L., Varty K., Allen K., Morgan J. D. T., Bell P. R. F., and London N.J.M. (1993) The histopathology of infrainguinal vein graft stenoses. Eur. J. Vasc. Surg. 7, 16–20.
Miller R. J. (1971) An elastin stain. Med. Lab. Tech. 28, 148–154.
Porter K. E., Varty K., Jones L., Bell P. R. F., and London N. J. M. (1996) Human saphenous vein organ culture: A useful model of intimal hyperplasia? Eur. J. Vasc. Endovasc. Surg. 11, 48–58.
Bobik A., Grooms A., Millar J. A., and Grinpukel S. (1990) Growth factor activity of endothelin on vascular smooth muscle. Am. J. Physiol. 258, C408–C415.
Hirata Y., Takagi Y., Fukuda Y., and Marumo F. (1989) Endothelin is a potent mitogen for rat vascular smooth muscle cells. Atherosclerosis 78, 225–228.
Masood I., Porter K. E., and London N. J. M. (1997) Endothelin-1 is a mediator of intimal hyperplasia in an organ culture of human saphenous vein. Br. J. Surg. 84, 499–503.
Arai H., Hori S., Aramori I., Ohkubo H., and Nakanishi S. (1990) Cloning and expression of a cDNA encoding an endothelin receptor. Nature 348, 730–732.
Sakurai T., Yanagisawa M., Takuwa Y., Miyazaki H., Kimura S., Goto K., and Masaki T. (1990) Cloning of a cDNA encoding a nonisopeptide-selective subtype of the endothelin receptor. Nature 348, 732–735.
Weber C., Schmitt R., Birnboeck H., Hopfgartner G., Vanmarle S. P., Peeters P. A. M., et al. (1996) Pharmacokinetics and pharmacodynamics of the endothelin receptor antagonist bosentan in healthy human subjects. Clin. Pharmacol. Ther. 60, 124–137.
Douglas S. A., Vickery-Clark L. M., Louden C., Elliot J. D., and Ohlstein E. H. (1995) Endothelin receptor subtypes in the pathogenesis of angioplasty-induced neointima formation in the rat-A comparison of selective ET(A receptor antagonism and dual ET(A)/ET(B) receptor antagonism using BQ123 and SB204670. J. Cardiovasc. Pharmacol. 26, S186–S189.
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Porter, K.E. (2002). Use of In Vitro Organ Cultures of Human Saphenous Vein as a Model for Intimal Proliferation. In: Maguire, J.J., Davenport, A.P. (eds) Peptide Research Protocols. Methods in Molecular Biology™, vol 206. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-289-9:199
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DOI: https://doi.org/10.1385/1-59259-289-9:199
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