Use of In Vitro Organ Cultures of Human Saphenous Vein as a Model for Intimal Proliferation
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The autologous internal mammary artery and the long saphenous vein are the most frequently used conduits as bypass grafts in the management of occlusive arterial disease in both the coronary and lower limb circulations. However, significant stenosis occurs in over a third of lower limb reconstructions in the first postoperative year (1), and the patency rate for coronary bypass grafts is only 50% after 5 yr (2). The underlying pathological lesion of such stenoses is intimal hyperplasia (IH). IH is characterized by excessive smooth muscle cell migration and proliferation in the intima of the vessel wall, together with an accumulation of extracellular matrix. This in turn results in a significant loss in lumenal area with a subsequent reduced blood flow to the tissues.
KeywordsOrgan Culture Saphenous Vein Intimal Hyperplasia Smooth Muscle Cell Proliferation Neointima Formation
- 3.Freshney R. I. (1987) Culture of Animal Cells, 2nd ed., Alan R. Liss Inc., New York, pp. 298–305.Google Scholar
- 13.Ihara M., Ishikawa K., Fukuroda T., Saeki T., Funabashi K., Fukami T., et al. (1992) In vitro biological profile of a highly potent novel endothelin (ET) antagonist BQ-123 selective for the ETA receptor. J. Cardiovasc. Pharmacol. 20, 511–514.Google Scholar
- 16.Miller R. J. (1971) An elastin stain. Med. Lab. Tech. 28, 148–154.Google Scholar
- 24.Douglas S. A., Vickery-Clark L. M., Louden C., Elliot J. D., and Ohlstein E. H. (1995) Endothelin receptor subtypes in the pathogenesis of angioplasty-induced neointima formation in the rat-A comparison of selective ET(A receptor antagonism and dual ET(A)/ET(B) receptor antagonism using BQ123 and SB204670. J. Cardiovasc. Pharmacol. 26, S186–S189.PubMedGoogle Scholar