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Characterization of a New Endothelin Receptor Ligand by In Vitro Assays

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Peptide Research Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 206))

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Abstract

Following the discovery of endothelin (1), functional characterization of the three endothelin (ET) isoforms (ET-1, ET-2 and ET-3) predicted that two mammalian receptor subtypes are present: the ETA receptor that is selective for ET-1, and the ETB receptor that has equal affinity for the three isoforms (2). The existence of the two distinct high-affinity ET receptor subtypes has been confirmed by cloning. Unique cDNAs that code for ETA and ETB belonging to the G-protein linked heptahelical receptor superfamily are identified in human, bovine and rat tissues (3–6). While pharmacological studies suggest that there may be more ET receptor subtypes (7), no additional homologous mammalian cDNAs have been identified.

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Author information

Authors and Affiliations

  1. Abbott Laboratories, Santa Clara, CA

    J. Ruth Wu-Wong

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  1. J. Ruth Wu-Wong
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Editor information

Editors and Affiliations

  1. Clinical Pharmacology Unit, University of Cambridge, Cambridge, UK

    Janet J. Maguire  & Anthony P. Davenport  & 

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© 2002 Humana Press Inc.

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Wu-Wong, J.R. (2002). Characterization of a New Endothelin Receptor Ligand by In Vitro Assays. In: Maguire, J.J., Davenport, A.P. (eds) Peptide Research Protocols. Methods in Molecular Biology™, vol 206. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-289-9:181

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  • DOI: https://doi.org/10.1385/1-59259-289-9:181

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-993-3

  • Online ISBN: 978-1-59259-289-0

  • eBook Packages: Springer Protocols

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