Characterization of a New Endothelin Receptor Ligand by In Vitro Assays

  • J. Ruth Wu-Wong
Part of the Methods in Molecular Biology™ book series (MIMB, volume 206)


Following the discovery of endothelin (1), functional characterization of the three endothelin (ET) isoforms (ET-1, ET-2 and ET-3) predicted that two mammalian receptor subtypes are present: the ETA receptor that is selective for ET-1, and the ETB receptor that has equal affinity for the three isoforms (2). The existence of the two distinct high-affinity ET receptor subtypes has been confirmed by cloning. Unique cDNAs that code for ETA and ETB belonging to the G-protein linked heptahelical receptor superfamily are identified in human, bovine and rat tissues (3, 4, 5, 6). While pharmacological studies suggest that there may be more ET receptor subtypes (7), no additional homologous mammalian cDNAs have been identified.


Binding Study Test Agent Arachidonic Acid Release Receptor Binding Study Coronary Artery Smooth Muscle Cell 
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Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  • J. Ruth Wu-Wong
    • 1
  1. 1.Abbott LaboratoriesSanta Clara

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