Analysis of Endothelins by Enzyme-Linked Immunosorbent Assay and Radioimmunoassay

  • Anthony P. Davenport
  • Rhoda E. Kuc
Part of the Methods in Molecular Biology™ book series (MIMB, volume 206)


This chapter describes procedures for the measurement of endothelin peptides by antibodies, focusing on the two main immunoassay techniques that are widely used. In a two-site “sandwich” enzyme-linked immunosorbent assay (ELISA) one antibody is immobilized to a solid phase and captures the endothelin (ET) peptide(s), which is quantified by the binding to this complex of a second, enzyme-labeled antibody in the liquid phase. In a radioimmunoassay (RIA), the ET peptide(s) to be measured competes for the binding of a fixed concentration of radiolabeled peptide to a fixed concentration of antibody in the liquid phase. In contrast to the ELISA, the immune complex measured in a RIA does not contain the analyte and therefore inverse (falling) standard curves of peptide concentration vs bound labeled peptide are produced. For a more detailed discussion of the two techniques, refs. 1 and 2 are recommended.


Brain Natriuretic Peptide Amersham Pharmacia Biotech Sandwich ELISAs Magnetic Rack Vasoactive Intestinal Contractor 
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Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  • Anthony P. Davenport
    • 1
  • Rhoda E. Kuc
    • 1
  1. 1.Clinical Pharmacology UnitUniversity of CambridgeCambridgeUK

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