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Characterization of Specialized mtDNA Glycosylases

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Mitochondrial DNA

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 197))

Abstract

Mitochondria are cytoplasmic organelles that generate cellular energy in the form of ATP by the process of oxidative phosphorylation. Mitochondria contain multiple copies of a 16.5-kb circular genome. Mitochondrial DNA (mtDNA) encodes a subset of 13 structural, 22 transfer (tRNA), and 2 ribosomal (rRNA) genes. These few polypeptides encoded by mtDNA are essential components of oxidative phosphorylation. The other proteins involved in the metabolism and processing of the mtDNA, including mtDNA regulatory factors, are encoded in the nuclear genome. Transcription of these genes takes place in the nucleus and translation in the cytoplasm is then followed by selective transport to the mitochondria.

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Author information

Authors and Affiliations

  1. Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, Baltimore, MD

    Simon G. Nyaga & Vilhelm A. Bohr

Authors
  1. Simon G. Nyaga
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  2. Vilhelm A. Bohr
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Editor information

Editors and Affiliations

  1. Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC

    William C. Copeland

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© 2002 Humana Press Inc.

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Nyaga, S.G., Bohr, V.A. (2002). Characterization of Specialized mtDNA Glycosylases. In: Copeland, W.C. (eds) Mitochondrial DNA. Methods in Molecular Biology™, vol 197. Humana Press. https://doi.org/10.1385/1-59259-284-8:227

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  • DOI: https://doi.org/10.1385/1-59259-284-8:227

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-972-8

  • Online ISBN: 978-1-59259-284-5

  • eBook Packages: Springer Protocols

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