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Measuring Oxidative mtDNA Damage and Repair Using Quantitative PCR

  • Janine H. Santos
  • Bhaskar S. Mandavilli
  • Bennett Van Houten
Part of the Methods in Molecular Biology™ book series (MIMB, volume 197)

Abstract

The human mitochondrial genome was completely sequenced in 1981 by Anderson and co-workers (1) and consists of a closed circular supercoiled DNA molecule of 16,569 base pairs. Mammalian cells characteristically contain a few hundred to several thousand mitochondria, each with 2-10 copies of the genome. The mitochondrial genome encodes 13 polypeptides, 22 transfer RNAs (tRNAs), and 2 rRNA. The 13 polypeptides encoded by the mitochondrial DNA (mtDNA) are essential subunits of the electron transport chain (ETC) and ATP synthase; cells lacking mtDNA are completely dependent on glycolysis for survival (2).

Keywords

Mitochondrial Genome Quantitative Polymerase Chain Reaction Quantitative Polymerase Chain Reaction Assay Human Mitochondrial Genome Base Excision Repair System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  • Janine H. Santos
    • 1
  • Bhaskar S. Mandavilli
    • 1
  • Bennett Van Houten
    • 1
  1. 1.Laboratory of Molecular Genetics, National Institute of Environmental Health SciencesNational Institutes of Health

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