Measuring mtDNA Mutation Rates in Saccharomyces cerevisiae Using the mtArg8 Assay

  • Micheline K. Strand
  • William C. Copeland
Part of the Methods in Molecular Biology™ book series (MIMB, volume 197)


Mitochondrial mutation rates have historically been more refractory to genetic analysis than nuclear mutation rates. This is the result of the lack of auxotrophic genes in the mitochondria, the multiple (50-100) copies of mitochondrial genomes per cell (1), the random segregation of mitochondrial organelles during meiotic and mitotic cell division, and the apparent unequal replication of mitochondria (2).


Mutation Rate Mitochondrial Genome Increase Copy Number Mitotic Cell Division Disruption Plasmid 
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Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  • Micheline K. Strand
    • 1
  • William C. Copeland
    • 2
  1. 1.Laboratory of Molecular GeneticsNational Institute of Environmental Health Sciences
  2. 2.Laboratory of Molecular GeneticsNational Institute of Environmental Health Sciences, National Institutes of Health

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