Reproducible Quantitative PCR of Mitochondrial and Nuclear DNA Copy Number Using the LightCycler™
Part of the
Methods in Molecular Biology™
book series (MIMB, volume 197)
Recent developments in PCR fluorimetry have allowed for quick quantification
of target molecules. Before the invention of fluorimetric quantitative PCR, researchers who wanted to quantify the amount of a gene in a sample did so painstakingly by limiting dilution, competitive polymerase chain reaction (PCR), or other methods, including high-performance liquid chromatography (HPLC), solid phase assays, dot blots, or immunoassay (1).
KeywordsPolymerase Chain Reaction Polymerase Chain Reaction Product Mitochondrial Gene Quantitative Polymerase Chain Reaction Polymerase Chain Reaction Buffer
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