Reproducible Quantitative PCR of Mitochondrial and Nuclear DNA Copy Number Using the LightCycler™

  • Alice Wong
  • Gino Cortopassi
Part of the Methods in Molecular Biology™ book series (MIMB, volume 197)


Recent developments in PCR fluorimetry have allowed for quick quantification

of target molecules. Before the invention of fluorimetric quantitative PCR, researchers who wanted to quantify the amount of a gene in a sample did so painstakingly by limiting dilution, competitive polymerase chain reaction (PCR), or other methods, including high-performance liquid chromatography (HPLC), solid phase assays, dot blots, or immunoassay (1).


Polymerase Chain Reaction Polymerase Chain Reaction Product Mitochondrial Gene Quantitative Polymerase Chain Reaction Polymerase Chain Reaction Buffer 
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Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  • Alice Wong
    • 1
  • Gino Cortopassi
    • 2
  1. 1.Department of Molecular BiosciencesUniversity of CaliforniaDavis
  2. 2.Molecular BiosciencesUniversity of CaliforniaDavis

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