Abstract
The reverse-transcriptase-polymerase chain reaction (RT-PCR) can be used to determine minute amounts of mRNAs in tissue samples by co-amplification of a quantified amount of a competitive sequence (internal standard), so-called quantitative competitive (qc) RT-PCR. The first description of qcRT-PCR was provided by Wang et al. (1). As an internal standard, an RNA template with the same primer sites as the target mRNA was reverse-transcribed. The amplified PCR products differed in size and were separated by agarose gel electrophoresis. The presence of 32P-labeled 5′ primer allowed quantification by scintillation counting.
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© 2002 Humana Press Inc.
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Greber-Platzer, S., Balcz, B., Fleischmann, C., Lubec, G. (2002). Using the Quantitative Competitive RT-PCR Technique to Analyze Minute Amounts of Different mRNAs in Small Tissue Samples. In: O’Connell, J. (eds) RT-PCR Protocols. Methods in Molecular Biology, vol 193. Humana Press. https://doi.org/10.1385/1-59259-283-X:029
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DOI: https://doi.org/10.1385/1-59259-283-X:029
Publisher Name: Humana Press
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