Part of the
Methods in Molecular Biology
book series (MIMB, volume 193)
The Basics of RT-PCR
Some Practical Considerations
The basic reverse-transcriptase-polymerase chain reaction (RT-PCR) technique is used routinely and widely in most biomedical research laboratories. The fundamental considerations for such basics as primer design, good laboratory set up and practice, and techniques for performing RT-PCR have been fully examined elsewhere (1,2). Thus, I will include only observations from my own experience in this chapter. Although the purpose of this chapter is to help the reader in setting up their own PCR assays, and to provide tips on effective PCR laboratory set up, it should be noted that each individual chapter in this volume contains complete experimental detail on the protocols described.
KeywordsOptimal Annealing Temperature Guanidine Thiocyanate Household Bleach Nonspecific Priming Cesium Chloride Density Gradient
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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