Abstract
The green fluorescent protein (GFP) was first observed in 1962, by Shimomura et al., as co-existing with the photoprotein, aequorin, purified from the bioluminescent jellyfish, Aequorea victoria (1a). Aequorin, activated through complex formation with Ca2+and the cofactor coelenterazine, emits blue light that is absorbed by GFP, a 238-amino acid polypeptide. In response to this energy, GFP emits green light via the excitation of its fluorophore.
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Eliopoulos, N., Galipeau, J. (2002). Green Fluorescent Protein in Retroviral Vector Constructs as Marker and Reporter of Gene Expression for Cell and Gene Therapy Applications. In: Hicks, B.W. (eds) Green Fluorescent Protein. Methods in Molecular Biology, vol 183. Humana Press. https://doi.org/10.1385/1-59259-280-5:353
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DOI: https://doi.org/10.1385/1-59259-280-5:353
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