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Green Fluorescent Protein inTransgenic Plants

Brassica Transformation

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Green Fluorescent Protein

Part of the book series: Methods in Molecular Biology ((MIMB,volume 183))

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Abstract

Until the heterologous expression of Aequorea victoria green fluorescent protein (GFP) was demonstrated, scientists working with transgenic organisms had no good alternative to using destructive visible genetic markers. Genes coding luciferase (1) and β-glucuronidase (2) are the most popular destructive marker genes that have been successfully used in transgenic plants. Although these markers code for sensitive enzymes that have linear dose responses, they require expensive substrates, and are limited to laboratory uses. Most of all, they cannot be used to assay living tissue directly.

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Author information

Authors and Affiliations

  1. Department of Plant Sciences and Landscape Systems, Ellington Plant Sciences, The University of Tennessee, Knoxville, TN

    C. Neal Stewart Jr., Matthew D. Halfhill & Reginald J. Millwood

  2. Department of Crop Science, Williams Hall, North Carolina State University, Raleigh, NC

    Matthew D. Halfhill

Authors
  1. C. Neal Stewart Jr.
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  2. Matthew D. Halfhill
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  3. Reginald J. Millwood
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Editor information

Editors and Affiliations

  1. Department of Chemistry, United States Air Force Academy, Colorado Springs, CO, USA

    Barry W. Hicks

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© 2002 Humana Press Inc.

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Stewart, C.N., Halfhill, M.D., Millwood, R.J. (2002). Green Fluorescent Protein inTransgenic Plants. In: Hicks, B.W. (eds) Green Fluorescent Protein. Methods in Molecular Biology, vol 183. Humana Press. https://doi.org/10.1385/1-59259-280-5:245

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  • DOI: https://doi.org/10.1385/1-59259-280-5:245

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-905-6

  • Online ISBN: 978-1-59259-280-7

  • eBook Packages: Springer Protocols

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