Abstract
Neurons in widely disparate regions of the central nervous system (CNS), regions not intimately associated with reproductive behaviors, express the estrogen gene. Recent in situ hybridization (ISH) studies have documented the widespread distribution of estrogen receptor mRNA in the adult rat brain (1–3). Clearly labeled cells were found in regions with widespread connections throughout the brain, suggesting that estrogen receptor may modulate a wide variety of neural functions. Furthermore, we reported the expression of estrogen receptor mRNA in bovine retina (4).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Pelletier, G., Liano, N., Follea, N., and Govindan, M. V. (1988) Mapping of estrogen receptor-producing cells in the rat brain by in situ hybridization. Neurosci Lett. 94, 23–28.
Toran-Allerand, C. D., Miranda, R. C., Hochberg, R. B., and MacLusky, N. J. (1992) Cellular variations in estrogen receptor mRNA translation in the developing brain: evidence from combined [125I] estrogen autoradiography and non-isotopic in situ hybridization histochemistry. Brain Res. 576, 25–41.
Simerly, R. B., Chang, C., Muramatsu, M., and Swanson, L. W. (1990) Distribution of androgen and estrogen receptor mRNA-containing cells in the rat brain: an in situ hybridization study. J. Comp. Neurol. 294, 76–95.
Kobayashi, K., Kobayashi, H., Ueda, M., and Honda, Y. (1998) Estrogen receptor expression in bovine and rat retinas. Invest. Ophthalmol. Vis. Sci. 39, 2105–2110.
Gee, C. E., Chen, C. L., Roberts, J. L., Thompson, R., and Watson, S. J. (1983) Identification of proopiomelanocortin neurones in rat hypothalamus by in situ cDNA-mRNA hybridization. Nature 306, 374–376.
Wisden W. and Morris, B. J. (1994) In Situ Hybridization Protocols for the Brain. Academic Press, New York.
Koji, T. and Brenner, R. M. (1993) Localization estrogen receptor messenger ribonucleic acid in rhesus monkey uterus by nonradioactive in situ hybridization with digoxigenin-labeled oligodeoxynucleotides. Endocrinology 132, 382–392.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2002 Humana Press Inc.
About this protocol
Cite this protocol
Kobayashi, K., Kobayashi, H. (2002). Detection of Estrogen Receptor by In Situ Hybridization. In: Armstrong, D. (eds) Oxidants and Antioxidants. Methods in Molecular Biology™, vol 196. Humana Press. https://doi.org/10.1385/1-59259-274-0:79
Download citation
DOI: https://doi.org/10.1385/1-59259-274-0:79
Publisher Name: Humana Press
Print ISBN: 978-0-89603-851-6
Online ISBN: 978-1-59259-274-6
eBook Packages: Springer Protocols