Abstract
One major source of reactive oxygen species (ROS) is the Fenton reaction: Fe2+ + H2O2 Fe3 + HO- + HO° Mass action implies that ROS generated by the Fenton reaction depend on the intracellular concentration of ferrous ions ([Fe2+]) although other metabolic fluxes make it difficult to capture this concentration dependence. Other metals such as Cu+may participate in a Fenton reaction and others may even have antioxidant activity; but, until recently, it was also difficult to affect intracellular [Fe2+] or the intracellular concentration of other divalent metals by experimental manipulation. This chapter reviews the discovery of a transporter that is involved in Fe2+flux and potentially in the flux of other divalent metals and describes some ways to manipulate expression of this transporter.
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© 2002 Humana Press Inc.
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Garrick, M.D., Dolan, K.G. (2002). An Expression System for a Transporter of Iron and Other Metals. In: Armstrong, D. (eds) Oxidants and Antioxidants. Methods in Molecular Biology™, vol 196. Humana Press. https://doi.org/10.1385/1-59259-274-0:147
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DOI: https://doi.org/10.1385/1-59259-274-0:147
Publisher Name: Humana Press
Print ISBN: 978-0-89603-851-6
Online ISBN: 978-1-59259-274-6
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