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The Immunohistochemical Localization of Glutathione Peroxidase

  • Kiyoshi Akeo
  • Tadahisa Hiramitsu
  • Keiichi Watanabe
Part of the Methods in Molecular Biology™ book series (MIMB, volume 196)

Abstract

Glutathione peroxidase (GSH-PO), a selenium-dependent and lipid peroxide-scavenging enzyme that effectively reduces lipid peroxides with the concomitant oxidation of glutathione is distributed in mitochondria (1,2). Utsunomiya et al. (3) confirmed the dual localization of GSH-PO in the cytosol and mitochondria of normal rat hepatocytes. We have shown that short-term incubation with linoleic acids (LA) increased the thiobarbituric acid- reactive substance (TBARS) in the RPE cells, which indicated the level of lipid peroxides (4). Mitochondria in the RPE cells were swollen by the incubation with LA or linoleic acid hydroperoxide (LHP) (5). We speculate that exposure of RPE cells to LA or LHP may cause damage to the mitochondria by lipid peroxidation, resulting in the cytotoxicity of RPE cells. We also found loss of mitochondria of bovine RPE cells cultured in hypoxia as low as 1% oxygen, induced malfunction of phagocytosis and a decrease in antioxidants such as glutathione containing sulfur (6).

Keywords

Sodium Dodecyl Sulfate Laser Scan Microscopy Outer Segment Photoreceptor Cell Subcellular Organelle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  • Kiyoshi Akeo
    • 1
  • Tadahisa Hiramitsu
    • 2
  • Keiichi Watanabe
    • 3
  1. 1.Department of OphthalmologyTakasaki National HospitalTakasaki-shiJapan
  2. 2.Photon Medical Research CenterHamamatsu University School of MedicineHamamatsuJapan
  3. 3.Department of PathologyTokai University School of Medicine, Boseidai Isehara-shiKanagawaJapan

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