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Activation of Nuclear Factor-κB (NF-κB) in Mononuclear Cells (MNC)

  • Ahmad Aljada
  • Husam Ghanim
  • Paresh Dandona
Part of the Methods in Molecular Biology™ book series (MIMB, volume 196)

Abstract

The transcription factor NF-κB was first identified as a regulator of the expression of the kappa light-chain gene in murine B lymphocytes (1). It has a central role in the immunological processes and regulates a wide range of genes in a rapid fashion (2). NF-κB interacts with its inhibitor protein IκB and activation of NF-κB causes it to move into the nucleus and this is controlled by the phosphorylation and subsequent degredation of IκB (3,4). The activated form of NF-κB is a heterodimer, which usually consists of two proteins, a p65 (relA) subunit and a p50 subunit. Other subunits (rel, relB, v-rel, and p52) can also bind to the activated NF-κB and thus can activate different sets of genes.

Keywords

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Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  • Ahmad Aljada
    • 1
  • Husam Ghanim
    • 1
  • Paresh Dandona
    • 1
  1. 1.Department of MedicineMillard Fillmore HospitalBuffalo

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