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PCR Mutation Detection Protocols pp 47–50Cite as

Allele-Specific Oligonucleotide PCR

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 187))

Abstract

Many single-base substitutions that lead to inherited diseases, the predisposition to genetic disorders, and cancer are increasingly being discovered. The ability to amplify specific DNA sequences by the polymerase chain reaction (PCR) (1) has made it possible to rapidly and accurately diagnose many inheritable diseases.

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References

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Author information

Authors and Affiliations

  1. Department of Psychiatry, Queen Elizabeth Psychiatric Hospital, University of Birmingham, Birmingham, UK

    Elaine K. Green

Authors
  1. Elaine K. Green
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Editor information

Editors and Affiliations

  1. Department of Haematology, Birmingham Children’s Hospital NHS Trust, Birmingham, UK

    Bimal D. M. Theophilus

  2. Department of Biosciences, University of Hertfordshire, Hatfield, UK

    Ralph Rapley

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© 2002 Humana Press Inc., Totowa, NJ

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Cite this protocol

Green, E.K. (2002). Allele-Specific Oligonucleotide PCR. In: Theophilus, B.D.M., Rapley, R. (eds) PCR Mutation Detection Protocols. Methods in Molecular Biology, vol 187. Humana Press. https://doi.org/10.1385/1-59259-273-2:047

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  • DOI: https://doi.org/10.1385/1-59259-273-2:047

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-617-8

  • Online ISBN: 978-1-59259-273-9

  • eBook Packages: Springer Protocols

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