Fluorescent In Situ Hybridization

  • Sara A. Dyer
  • Elaine K. Green
Part of the Methods in Molecular Biology book series (MIMB, volume 187)


Single-stranded DNA will recognize a complementary strand with high specificity under suitably controlled conditions. In situ hybridization (ISH) exploits this phenomenon by hybridizing an appropriately labeled singlestranded DNA “probe” to target sequences in situ in either dissociated cell preparations or tissue sections.


Rubber Sealant Hybridization Chamber Centromeric Probe Denaturation Solution Target Slide 
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  1. 1.
    Speicher, M. R., Ballard, S. G., and Ward, D. C. (1996) Karyotyping human chromosomes by combinatorial multi-fluor FISH. Nature Genet. 12, 368–375.PubMedCrossRefGoogle Scholar
  2. 2.
    Kallioniemi, A., Kallioniemi O-P., Sudar, D., Rutovitz, D. Gray, J. W., Waldman F., et al. (1992) Comparative genomic hybridization for molecular cytogenetic analysis of solid tumours. Science 258, 18–821.CrossRefGoogle Scholar
  3. 3.
    Lichter, P., Cremer, T., Borden, J., Manuelidis, L., and Ward, D. C. (1988) Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries. Hum. Genet. 80, 224–234.PubMedCrossRefGoogle Scholar
  4. Barch, M. J., Knutsen, T., and Spurbeck, J., eds. (1997) The AGT Cytogenetics Laboratory Manual,.Lippincott-Raven, PA.Google Scholar
  5. Rooney, D. E. and Czepulkowski, B. H., eds. (1992) Human Cytogenetics, Volume I, IRL, Oxford.Google Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Sara A. Dyer
    • 1
  • Elaine K. Green
    • 2
  1. 1.Regional Genetics LaboratoryBirmingham Women’s HospitalBirminghamUK
  2. 2.Department of Psychiatry, Queen Elizabeth Psychiatric HospitalUniversity of BirminghamBirminghamUK

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