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Fluorescent In Situ Hybridization

  • Sara A. Dyer
  • Elaine K. Green
Part of the Methods in Molecular Biology book series (MIMB, volume 187)

Abstract

Single-stranded DNA will recognize a complementary strand with high specificity under suitably controlled conditions. In situ hybridization (ISH) exploits this phenomenon by hybridizing an appropriately labeled singlestranded DNA “probe” to target sequences in situ in either dissociated cell preparations or tissue sections.

Keywords

Rubber Sealant Hybridization Chamber Centromeric Probe Denaturation Solution Target Slide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Sara A. Dyer
    • 1
  • Elaine K. Green
    • 2
  1. 1.Regional Genetics LaboratoryBirmingham Women’s HospitalBirminghamUK
  2. 2.Department of Psychiatry, Queen Elizabeth Psychiatric HospitalUniversity of BirminghamBirminghamUK

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