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Part of the book series: Methods in Molecular Medicineā„¢ ((MIMM,volume 72))

Abstract

This chapter describes methods relating to the handling and processing of Anopheles mosquitoes captured during malaria vector field studies. Areas covered include the following methods

  1. 1.

    Handling mosquitoes in the field.

  2. 2.

    Developing assembly-line approaches for specimen processing.

  3. 3.

    Taxonomically identifying mosquitoes.

  4. 4.

    Classifying blood-feeding stages, parity, and Christophersā€™ stages of ovarian development.

  5. 5.

    Using molecular methods for identifying specific species of mosquitoes belonging to Anopheles species complexes.

This chapter complements Chapter 1.

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References

  1. WHO (1975) Manual on Practical Entomology in Malaria. Part II. Methods and Techniques. WHO Offset Publication 13, Geneva.

    Google ScholarĀ 

  2. Beier, J. C. (1996) requent blood-feeding and restrictive sugar-feeding behavior enhance the malaria vector potential of Anopheles gambiae s.l. and An. funestus (Diptera: Culicidae) in western Kenya. J. Med. Entomol. 33, 613ā€“618.

    CASĀ  PubMedĀ  Google ScholarĀ 

  3. Lyimo, E. O. and Takken, W. (1993) Effects of adult body size on fecundity and the pre-gravid rate of Anopheles gambiae females in Tanzania. Med. Vet. Entomol. 7, 328ā€“332.

    ArticleĀ  CASĀ  PubMedĀ  Google ScholarĀ 

  4. Gillies, M. T. and DeMeillon, B. (1968) The Anophelinae of Africa South of the Sahara. The South African Institute for Medical Research, Johannesburg.

    Google ScholarĀ 

  5. Service, M. W. (1976) Mosquito Ecology. Wiley, New York.

    Google ScholarĀ 

  6. Service, M. W. (1993) Mosquito Ecology: Field Sampling Methods. Elsevier Applied Science, New York.

    Google ScholarĀ 

  7. Briegel, H. and Horler, E. (1993) Multiple blood meals as a reproductive strategy in Anopheles (Diptera: Culicidae). J. Med. Entomol. 30, 975ā€“985.

    CASĀ  PubMedĀ  Google ScholarĀ 

  8. Klowden, M. J. and Chambers, G. M. (1992) Reproductive and metabolic differences between Aedes aegypti and A. albopictus (Diptera: Culicidae). J. Med. Entomol. 29, 467ā€“471.

    CASĀ  PubMedĀ  Google ScholarĀ 

  9. Klowden, M. J. and Briegel, H. (1994) Mosquito gonotrophic cycle and multiple feeding potential: contrasts between Anopheles and Aedes (Diptera: Culicidae). J. Med. Entomol. 31, 618ā€“622.

    CASĀ  PubMedĀ  Google ScholarĀ 

  10. Coluzzi, M., Sabatini, A., Petrarca, V., and Di Deco, M. A. (1979) Chromosomal differentation and adaptation to human environments in the Anopheles gambiae complex.Trans. R. Soc. Trop. Med. Hyg., 73, 483ā€“497.

    ArticleĀ  CASĀ  Google ScholarĀ 

  11. Scott, J. A., Brogdon, W. G., and Collins, F. H. (1993) Identification of single specimens of the Anopheles gambiae complex by the polymerase chain reaction. Am. J. Trop. Med. Hyg. 49, 520ā€“529.

    CASĀ  PubMedĀ  Google ScholarĀ 

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Ā© 2002 Humana Press, Inc., Totowa, NJ

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Beier, J.C. (2002). Vector Analysis. In: Doolan, D.L. (eds) Malaria Methods and Protocols. Methods in Molecular Medicineā„¢, vol 72. Humana Press. https://doi.org/10.1385/1-59259-271-6:95

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  • DOI: https://doi.org/10.1385/1-59259-271-6:95

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-823-3

  • Online ISBN: 978-1-59259-271-5

  • eBook Packages: Springer Protocols

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