Abstract
To determine the immunogenicity of vaccine candidates, it is essential to establish an accurate and convenient methodology for assessing the level of antigen-specific T-cell responses, and several methodologies have been developed. These include the T-cell proliferative assay as determined by tritium-labeled thymidine (3H-TdR) incorporation, cytotoxic assays such as the chromium release assay, cytokine enzyme-linked immunosorbent assays (ELISAs) or the T-cell enzyme-linked immunospot (ELISPOT) assay to determine the amounts of cytokines released by activated T cells, and most recently, the newly developed tetramer assay. All these methods, each of which have both advantages and disadvantages, have been used to measure the level of cell-mediated immune responses in animals and humans infected with microbes or immunized with microbial antigens or various vaccines.
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Tsuji, M. (2002). Assessing Antigen-Specific CD8+ and CD4+ T-Cell Responses in Mice After Immunization with Recombinant Viruses. In: Doolan, D.L. (eds) Malaria Methods and Protocols. Methods in Molecular Medicineā¢, vol 72. Humana Press. https://doi.org/10.1385/1-59259-271-6:361
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DOI: https://doi.org/10.1385/1-59259-271-6:361
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