Abstract
Subtractive hybridization techniques are designed to deplete one cDNA population (the “target”) of sequences common to a second group of cDNAs (the “driver”), thereby effectively enriching the target population for unique sequences. The applications of this powerful methodology are widespread, and have included identification of genes expressed during embryonic development as well as genes expressed solely in specialized regions of the brain (1-5).
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© 2002 Humana Press Inc.
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Fidock, D.A., Nguyen, T.V., Beerntsen, B.T., James, A.A. (2002). Production of Stage-Specific Plasmodium falciparum cDNA Libraries Using Subtractive Hybridization. In: Doolan, D.L. (eds) Malaria Methods and Protocols. Methods in Molecular Medicine™, vol 72. Humana Press. https://doi.org/10.1385/1-59259-271-6:277
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DOI: https://doi.org/10.1385/1-59259-271-6:277
Publisher Name: Humana Press
Print ISBN: 978-0-89603-823-3
Online ISBN: 978-1-59259-271-5
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