Abstract
Plasmodium berghei was probably first described in 1946 by Vincke in blood films of the stomach contents of Anopheles dureni. In 1948, it was subsequently found in blood films of Grammomys surdaster collected in Kisanga, Katanga; blood was passaged to white rats and became the K173 strain made widely available by the Institute for Tropical Medicine in Antwerp. A trio of fascinating papers describing the discovery and early analysis of the biology of P. berghei (1-3) give an early indication of the natural and catholic host range of this parasite (Table 1), a property that possibly underlies the successful transmission of the parasite to a variety of laboratory hosts. Although the natural vector of P. berghei is A. dureni, early laboratory studies (3,4) showed that a wide range of colonized mosquitoes would successfully transmit the parasite (Table 2).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
van den Berghe, L. (1954) The history of the discovery of Plasmodium berghei. Indian J. Malariol. 8, 241ā243.
Vincke, I. H. (1954) Natural history of Plasmodium berghei. Indian J. Malariol. 8, 245ā256.
Yoeli, M. (1965) Studies on Plasmodium berghei in nature and under experimental conditions. Trans. R. Soc. Trop. Med. Hyg. 59, 255ā276.
Bray, R. S. (1954) The mosquito transmission of Plasmodium berghei. Indian J. Malariol. 8, 263ā274.
Killick-Kendrick, R. (1978) Taxonomy, zoogeography and evolution, in Rodent Malaria (Killick-Kendrick, R. and Peters, W., eds.), Academic Press, London, pp. 1ā52.
Bafort, J. (1968) The effects of low temperature preservation on the viability of sporozoites of Plasmodium berghei. Ann. Trop. Med. Parasitol. 62, 301ā304.
Collins, W. E., Morris, C. L., Richardson, B. B., Sullivan, J. S., and Galland, G. G. (1994) Further studies on the sporozoite transmission of the Salvador I strain of Plasmodium vivax. J. Parasitol. 80, 512ā517.
Hollingdale, M. R., Leland, P., Sigler, C. I., and Leef, J. L. (1985). In vitro infectivity of cryopreserved Plasmodium berghei sporozoites to cultured cells. Trans. R. Soc.Trop. Med. Hyg. 79, 206ā208.
Walliker, D. and Beale, G. (1993) Synchronization and cloning of malaria parasites. Methods Mol. Biol. 21, 57ā66.
Fowler, R. E., Sinden, R. E., and Pudney, M. (1995) Inhibitory activity of the anti-malarial Atovaquone (566C80) against ookinetes, oocysts, and sporozoites of Plasmodium berghei. J. Parasitol. 81, 452ā458.
Shmuklarsky, M. J., Klayman, D. L., Milhous, W. K., Kyle, D. E., Rossan, R. N., Ager, A. L., et al. (1993) Comparison of beta-Artemether and beta-Arteether against malaria parasites in vitro and in vivo. Am. J. Trop. Med. Hyg. 48,.377ā384.
Sinden, R. E. (1996) Infection of mosquitoes with rodent malaria, in Molecular Biology of Insect Disease Vectors: A Methods Manual (Crampton, J. M., Beard, C. B., and Louis, C., eds.), Chapman and Hall, London, pp. 67ā91.
Sinden, R. E., Suhrbier, A., Davies, C. S., Fleck, S. L., Hodivala, K., and Nicholas, J. C. (1990) The development and routine application of high-density exoerythrocytic-stage cultures of Plasmodium berghei. Bull.WHO 68, 115ā125.
Mons, B. (1986) Intraerythrocytic differentiation of Plasmodium berghei. Acta Leidensia 54, 1ā83.
Sinden, R. E., Hartley, R. H., and Winger, L. (1985) The development of Plasmodium ookinetes in vitro: an ultrastructural study including a description of meiotic division. Parasitol. 91, 227ā244.
Janse, C. J., Mons, B., Rouwenhorst, R. J., Klooster Van Der, P. F. J., Overdulve, J. P., and Kaay Van Der, H. J. (1985) In vitro formation of ookinetes and functional maturity of Plasmodium berghei gametocytes. Parasitol. 91, 19ā29.
Sinden, R. E. and Smalley, M. E. (1976) Gametocytes of Plasmodium falciparum: phagocytosis by leucocytes in vivo and in vitro. Trans. Roy. Soc. Trop. Med. Hyg. 70, 344,345.
Janse, C. J., Camargo, A., Delportillo, H. A., Herrera, S., Waters, A. P., Kumlien, S., Mons, B., and Thomas, A. (1994). Removal of leucocytes from Plasmodium vivax infected blood. Ann.Trop.Med.Parasitol. 88, 213ā216.
Beetsma, A. L., Vandewiel, T. J. J. M., Sauerwein, R. W., and Eling, W. M. C. (1998). Plasmodium berghei ANKA: purification of large numbers of infectious gametocytes. Exp. Parasitol. 88, 69ā72.
Ozaki, L. S., Gwadz, R. W., and Godson, G. N. (1984) Simple centrifugation method for rapid separation of sporozoites from mosquitoes. J. Parasitol. 70, 831ā833.
Sinden, R. E. and Croll, N. A. (1975) Cytology and kinetics of microgametogenesis and fertilization of Plasmodium yoelii nigeriensis. Parasitol. 70, 53ā65.
Janse, C. J. and Waters, A. P. (1995) Plasmodium berghei: the application of cultivation and purification techniques to molecular studies of malaria parasites. Parasitol. Today 11, 138ā143.
Al-Olayan, E., Beetsma, A. L., Butcher, G. A., Sinden, R. E., and Hurd, H. (2002) Complete development of mosquito phases of the malaria parasite in vitro. Science, 295, 677ā679.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
Ā© 2002 Humana Press Inc.
About this protocol
Cite this protocol
Sinden, R.E., Butcher, G.A., Beetsma, A.L. (2002). Maintenance of the Plasmodium berghei Life Cycle. In: Doolan, D.L. (eds) Malaria Methods and Protocols. Methods in Molecular Medicineā¢, vol 72. Humana Press. https://doi.org/10.1385/1-59259-271-6:25
Download citation
DOI: https://doi.org/10.1385/1-59259-271-6:25
Publisher Name: Humana Press
Print ISBN: 978-0-89603-823-3
Online ISBN: 978-1-59259-271-5
eBook Packages: Springer Protocols